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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Supplier: MP Biomedicals

Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.

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Phosphate Buffer Mixed pH 4,0

Supplier: CPACHEM

Shelf Life on Ship Date: 12 months - Store under normal laboratory conditions, at temperatures between 15 to 25 °C.

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pH buffers, ready to use

pH buffers, ready to use

Supplier: WTW

Every bottle has its own lot reference with the expiration date.

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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

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STERI-BUFFER

STERI-BUFFER

Supplier: VELTEK ASSOCIATES

Developed for use as a buffer solution and diluent in lab testing such a bioburden testing, pharmaceutical microbial limits testing, and AET testing and as a rinsing agent.

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Buffer solutions, colour coded, certified (20 °C), Fluka™

Supplier: Honeywell Chemicals

Phosphate, pH value: 7.00, 6.993 - 7.007, Reference temperature: 20 °C, Green

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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Buffer solutions

Buffer solutions

Supplier: SI Analytics

Buffer solutions intended for extremely precise calibration.

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T-PER™ Tissue Protein Extraction Reagent

T-PER™ Tissue Protein Extraction Reagent

Supplier: Thermo Fisher Scientific

Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

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Protein extraction reagent, BugBuster® 10X, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

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TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations

TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations

Supplier: Mettler - Toledo

TISAB III solution for fluoride determinations

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Tris Hydrochloride

Supplier: Merck Millipore (Calbiochem‎)

Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).

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Hank's balanced salt solution, modified, MP Biomedicals

Supplier: MP Biomedicals

Cell culture buffer, Hank's balanced salt solution, modified (1X)

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pH electrode calibration and maintenance solutions kit

pH electrode calibration and maintenance solutions kit

Supplier: WTW

Adapted to different electrode types for calibration and maintenance.

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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Used to minimise cell attachment or aggregation.

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pH Electrode Buffers and Solutions

Supplier: Cole-Parmer

pH buffer solution, pH value: 7, Sachet

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pH buffers

pH buffers

Supplier: Cole-Parmer

Available as single use sachets or as 500 ml bottles.

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Hank's balanced salts, MP Biomedicals

Supplier: MP Biomedicals

Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

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Saline solutions

Supplier: BALIS

Cell culture buffer, Physiological saline solution, 9 ml, no standards

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: SERACARE LIFE SCIENCES

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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pH buffer solutions

pH buffer solutions

Supplier: Mettler - Toledo

Technical buffer solution, 6×250 ml, pH value: 7,00

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Buffer tablets, Lovibond®

Supplier: Lovibond

Ideal for preparing buffer solutions of 4, 7 and 10 pH units.

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pH buffer solutions, NIST/DIN

Supplier: Mettler - Toledo

The NIST/DIN pH buffers are specified to three decimal places, e.g. 9.180, offering the highest possible pH precision.

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Ammonia - Ammonium Chloride Buffer Solution, pH 10

Supplier: CPACHEM

Ammonia - Ammonium chloride buffer solution.

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pH buffers, for LAQUAtwin meters

Supplier: HORIBA

pH 4,01 buffer (6× 14 ml bottles per pack), Bottle

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pH buffer solutions, certified (DKD)

Supplier: Mettler - Toledo

DKD certified buffer solution, pH value: 9,21, 1× 250 ml

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pH Buffer solutions, CX-1

pH Buffer solutions, CX-1

Supplier: Thermo Fisher Scientific

Delivers robust reproducible gradients with the Thermo Scientific™ pH buffer platform that is applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

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pH buffer solutions, NIST traceable, Orion™

pH buffer solutions, NIST traceable, Orion™

Supplier: Thermo Orion

Perform accurate, reproducible pH calibrations with Orion™ pH buffers.

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Redox buffer solutions

Supplier: Mettler - Toledo

Redox buffer solutions are used for verification of all common redox sensors.

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