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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

ES Buffer

ES Buffer

Supplier: FUJIFILM WAKO CHEMICALS

The ES Buffer is an endotoxin-specific buffer that is ideal for untreated LAL.

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VWR® Transfer Buffer Concentrate, CAPS 10X

VWR® Transfer Buffer Concentrate, CAPS 10X

Supplier: VWR Chemicals

For transfer of protein to membranes for sequencing or blotting.

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: PanReac AppliChem

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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VWR®, Blocking buffer, non-fat powdered milk

VWR®, Blocking buffer, non-fat powdered milk

Supplier: VWR Chemicals

Each pack of powdered blocking buffer makes one liter of 1X solution.

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Phosphate buffered saline (PBS), 10X

Phosphate buffered saline (PBS), 10X

Supplier: G-Biosciences

PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

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Blocking buffers, FirstChoice™

Blocking buffers, FirstChoice™

Supplier: G-Biosciences

FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

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Trizmal™ Buffer, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

For use in the Naphthol AS-D Chloroacetate Esterase Kit No. 91-C.

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RNA isolation buffers

Supplier: OMEGA BIO-TEK

RNA Wash Buffer I

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MMP-1 assay buffer

Supplier: ENZO LIFE SCIENCES

MMP-1 assay buffer

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VWR® Separating Gel Buffer, 4X

VWR® Separating Gel Buffer, 4X

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.

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VWR®, MOPS 10X buffer for biotechnology

VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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VWR® Proteomics Grade, Tris-glycine buffer

VWR® Proteomics Grade, Tris-glycine buffer

Supplier: VWR Chemicals

A pre-blended Tris-glycine powder for preparation of Laemmli SDS-PAGE running buffer. The 40 litre pack size prepares 40 litres of 1X Tris-glycine solution (0,025 M Tris base and 0,192 M glycine). Each Ready-Pack™ prepares 1 litre of a 10X concentrate.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: G-Biosciences

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

Supplier: Thermo Fisher Scientific

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Buffer, TRIS solution pH 8 (1 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/L)

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Buffers, TE solutions 10X concentrates (TRIS-EDTA buffer)

Buffers, TE solutions 10X concentrates (TRIS-EDTA buffer)

Supplier: Rockland Immunochemicals

TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.

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Wash buffer concentrate

Supplier: ENZO LIFE SCIENCES

Liquid. TRIS buffered saline containing detergents.

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Proteasome assay buffer

Supplier: ENZO LIFE SCIENCES

Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.

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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

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Plasmid DNA buffers

Supplier: OMEGA BIO-TEK

Solution 1, resuspension buffer

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Buffer, PBS (10X, Dulbecco's) powder pack

Buffer, PBS (10X, Dulbecco's) powder pack

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (10X) according to Dulbecco.

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Supplier: Rockland Immunochemicals

EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.

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Protein blocking buffers, Superior™

Protein blocking buffers, Superior™

Supplier: G-Biosciences

Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.

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Buffer HF-Improved

Supplier: Thermo Fisher Scientific

Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.

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GET buffer

Supplier: Rockland Immunochemicals

GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.

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Protein Transfer buffers

Supplier: G-Biosciences

Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.

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WB blocking buffer kit, TrueBlack®

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

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Cathepsin S Assay Buffer

Supplier: ENZO LIFE SCIENCES

50mM potassium phosphate buffer, pH 6,5, 50 mM NaCl, 2 mM EDTA, 0,01% Triton X-100®, 0,5 mM DTT.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Rockland Immunochemicals

This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

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RIPA cell lysis buffer 2

Supplier: ENZO LIFE SCIENCES

RIPA cell lysis buffer 2, Enzo Life Sciences

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