You searched for: Nucleic Acid Purification Kits and Reagents

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® SP plant DNA kit is specially designed for the rapid and reliable isolation of high quality total cellular DNA from a variety of plant species. Up to 100 mg of fresh samples or 30 mg of dry samples can be processed in less than 40 minutes. Purified DNA is suitable for most downstream applications including PCR, restriction digestions and hybridisation techniques.

Supplier: Copan

NUCLEIC-CARD™ is specifically designed to collect, transport, and store human DNA from buccal cells, saliva, blood, etc. Thanks to the unique chemical treatment, NUCLEIC-CARD™ preserves DNA for 20 years at room temperature.

Supplier: AGILENT

The StrataPrep PCR purification kits provide a rapid method to simultaneously purify PCR products from PCR primers, unincorporated nucleotides, buffer components, and enzymes.

Supplier: Enzo Life Sciences

Rapid and complete removal of gDNA from RNA preps prior to reverse transcription.

Supplier: OMEGA BIO-TEK

Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.

Supplier: AGILENT

StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible.

Supplier: Merck

CHEMICON's EX-WAX™ DNA Extraction Kit is intended to extract DNA from paraffin-embedded tissue fixed in 10% Formalin or non-crosslinking fixatives. DNA extracted by this kit is suitable for amplification by PCR.

Supplier: OMEGA BIO-TEK

Isolate DNA from tissues, buccal swabs, cultured cells, whole blood, body fluids, paraffin-embedded tissues, and mouse tail snips using mini spin columns.

Supplier: Corning

The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Supplier: OMEGA BIO-TEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Supplier: OMEGA BIO-TEK

High-throughput DNA and RNA isolation from cultured cells and tissue using magnetic beads.

Supplier: OMEGA BIO-TEK

E-Z Select® 24-well Plate, 25 ml is designed for performing large volume applications on automated platforms such as Hamilton Microlab® STAR or equivalent.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® Fastfilter® Plasmid kits rapidly purify plasmid DNA utilising lysate clearance filter syringes, whilst E-Z 96® Fastfilter Plasmid kits employ lysate clearance plates in a 96-well format. Fastfilter® kits allow midi- and maxi-scale plasmid isolation in less than 40 m.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Universal Pathogen Kit allows for the rapid and reliable isolation of high quality host genomic DNA, gram positive and negative bacterial DNA, fungal spore DNA and viral DNA and viral RNA from tissue, urine, serum and fecal samples.

Supplier: G-Biosciences

The GET™ Total RNA Kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA spin columns. After homogenisation, RNA is bound to the GET™ RNA spin columns and washed. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays.

Supplier: AGILENT

RNA isolation kits provide a straightforward, spin-column method to deliver RNA for use in gene expression and other downstream analysis.

Supplier: OMEGA BIO-TEK

Isolate DNA from molluscs and insects using spin columns.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Stool DNA kit allows rapid and reliable isolation of high quality total DNA from fresh and frozen stool samples. Up to 200 mg of stool samples can be processed in less than 60 minutes. The system combines the reversible nucleic acid-binding properties of HiBind matrix with the speed and versatility of spin column technology to eliminate humic acid, polysaccharides, phenolic compounds, and enzyme inhibitors from stool samples. Purified DNA is suitable for PCR, restriction digestion, and hybridisation techniques.

Supplier: OMEGA BIO-TEK

Sequentially isolate DNA and RNA in two separate eluates from the same FFPE sample.

Supplier: Enzo Life Sciences

PCR/Gel Clean Up Kits are quick and simple kits for purifying DNA from PCR, gels and labelling reactions for a variety of downstream applications.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® Plant RNA kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogeniser column for filtration and homogenisation of viscous plant cell lysate by centrifugation in combination with the HiBind® RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, northern blot analysis, differential display, and poly(A)+ RNA selection.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Stool DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (both pathogen and host DNA) from stool samples.

Supplier: OMEGA BIO-TEK

Automation-ready, prefilled 96-well plates for quick and convenient DNA extraction from blood, saliva, cultured cells, and swabs for magnetic processors.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Bacterial DNA kit allows rapid and reliable isolation of high quality genomic DNA (gDNA) from a wide variety of bacterial species. Up to 0,5 ml gram positive or gram negative bacterial culture can be processed each time. The key to the system are the Mag-Bind® particles that avidly, but reversibly, bind DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed.

Supplier: OMEGA BIO-TEK

Hi-Bind® DNA mini columns are silica glass fibre columns that can bind up to 100 μg of genomic DNA or 35 μg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation.

Supplier: OMEGA BIO-TEK

The E-Z 96® Plant DNA DS Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Blood DNA HV Kit CE IVD is designed for rapid and reliable isolation of high-quality genomic DNA from up to 4 ml whole blood samples.

Supplier: OMEGA BIO-TEK

Selected protease digestion releases microgram amounts of DNA from FFPE samples.