You searched for: Nucleic Acid Purification Kits and Reagents

Supplier: OMEGA BIO-TEK

The Mag-Bind® Plant DNA 96 plus kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of difficult to lyse and high polysaccharide plant species and tissues. The system utilises a CTAB-based lysis buffer without the need for a chloroform or other organic solvent steps. The proprietary wash buffer systems eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. The Mag-Bind® Plant DNA plus kit also provides greater flexibility than silica plate based systems as partial plates can be used and can be scaled up to a 24-well format with the appropriate magnetic stand. The streamlined protocol can be easily adapted on most robotic liquid handling platforms. Purified DNA is suitable for PCR, qPCR, restriction digestion, hybridisation applications, as well as more demanding applications, such as RAPD.

Supplier: OMEGA BIO-TEK

Pre-scripted solution for purification of high-quality DNA from blood, saliva, cultured cells, or fresh or frozen tissue.

Supplier: Merck

Plasmid Miniprep 96 kit is a quick and easy-to-use kit that produces clean and reproducible DNA in half a time than traditional methods.

Supplier: OMEGA BIO-TEK

Isolate total RNA from cells or soft tissues using spin column.

Supplier: OMEGA BIO-TEK

MicroElute® Clean Up system, designed for rapid DNA clean up.

Supplier: Copan

NAO® Basket for rapid and efficient DNA recovery. This is a semi-permeable system designed for releasing and concentrating human DNA from swab samples or other specimens during the extraction step. Lysis in the NAO® Basket: NAO® Basket retains the lysis buffer during the lysis step of the forensic sample.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® blood DNA kit provides rapid total DNA isolation from up to 250 µl of fresh or frozen anticoagulated whole blood. The E.Z.N.A.® blood DNA kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® blood DNA method is ready for applications such as PCR, southern blotting, or restriction enzyme digestion.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and invitro translation.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® tissue DNA kit offers a simple, rapid, and cost effective method for the isolation of DNA from a wide variety of sample sources including fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in less than 30 minutes. Up to 30 mg of tissue at a time can be readily processed at a time using the simple E.Z.N.A.® tissue DNA protocol. Single or multiple samples can be simultaneously processed with this spin-column based kit. There is no need for phenol/chloroform extractions, or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.® tissue DNA kit is ready for most downstream applications such as PCR, southern blot and restriction enzyme digestion.

Supplier: OMEGA BIO-TEK

RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

Supplier: Rockland Immunochemicals

The set includes five unlabelled ModDetect™ phosphorothioate detection reagents, curated by binding characteristics, and three reporter molecules labelled with peroxidase, 488 λ fluorophore and 649 λ fluorophore.

Supplier: Enzo Life Sciences

EPIXTRACT® DNA isolation kit provides a simple method to isolate DNA from plasma, serum, and body fluids.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Food DNA Kit allows rapid and reliable isolation of high quality DNA from complex matrixes such as processed food, chocolate, cereals and meat.

Supplier: Quantabio

Rapid extraction and purification of high-quality total RNA from cultured cells or tissue.

Supplier: OMEGA BIO-TEK

Isolate DNA from plant or fungal samples using spin columns.

Supplier: OMEGA BIO-TEK

Isolate host genomic, bacterial, fungal spore, and viral DNA and viral RNA from tissue, urine, serum, and fecal samples using magnetic beads.

Supplier: Corning

The AxyPrep™ Mag PCR Normalizer Kit utilizes a paramagnetic bead-based purification system for PCR clean up.

Supplier: Merck

The device utilises gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

Supplier: OMEGA BIO-TEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

Supplier: OMEGA BIO-TEK

Gel purification of DNA is a common technique used for the isolation of specific DNA fragments. However, most methods either fail to completely remove agarose (which can lead to problems in downstream manipulations), shear the DNA, or result in very low yields.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Forensic DNA Kit is designed to provide a rapid and easy method for the isolation of genomic DNA from forensic samples such as dry blood, buccal swabs, and semen samples for consistent PCR and Southern analysis. This kit can also be used for the preparation of genomic DNA from mouse tail snips, whole blood, buffy coat, serum, and plasma.

Supplier: OMEGA BIO-TEK

Purify endotoxin free (<0,1 EU/µg) plasmid DNA using magnetic beads from up to 50 ml culture volume.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Bacterial RNA kit is designed for isolation of high-quality total RNA from variety of bacterial strains. Up to 1×10⁹ log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridisation techniques.

Supplier: Corning

Removing excess dye terminator is an essential step prior to Sanger sequencing. Carryover of the excess dye into the sequencing reactions may result in dye blobs resulting in inaccurate results. The AxyPrep™ Mag DyeClean kit utilises a paramagnetic bead-based purification system for Sanger sequencing reaction dye terminator clean-up. The protocol is simple and comprises binding, washing and elution steps which can be performed directly in the thermal cycling plate. This kit requires no centrifugation or filtration steps.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® SP plant DNA kit is specially designed for the rapid and reliable isolation of high quality total cellular DNA from a variety of plant species. Up to 100 mg of fresh samples or 30 mg of dry samples can be processed in less than 40 minutes. Purified DNA is suitable for most downstream applications including PCR, restriction digestions and hybridisation techniques.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.

Supplier: Corning

The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Supplier: AGILENT

Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast.