You searched for: Nucleic Acid Purification Kits and Reagents

Supplier: OMEGA BIO-TEK

This second generation plasmid miniprep kit yields 40 to 70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 ml to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.

Supplier: OMEGA BIO-TEK

High-throughput DNA and RNA isolation from cultured cells and tissue using magnetic beads.

Supplier: OMEGA BIO-TEK

E-Z Select® 24-well Plate, 25 ml is designed for performing large volume applications on automated platforms such as Hamilton Microlab® STAR or equivalent.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® Fastfilter® Plasmid kits rapidly purify plasmid DNA utilising lysate clearance filter syringes, whilst E-Z 96® Fastfilter Plasmid kits employ lysate clearance plates in a 96-well format. Fastfilter® kits allow midi- and maxi-scale plasmid isolation in less than 40 m.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Universal Pathogen Kit allows for the rapid and reliable isolation of high quality host genomic DNA, gram positive and negative bacterial DNA, fungal spore DNA and viral DNA and viral RNA from tissue, urine, serum and fecal samples.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1×10⁷ eukaryotic cells or 25 to 30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Supplier: G-Biosciences

The GET™ Total RNA Kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA spin columns. After homogenisation, RNA is bound to the GET™ RNA spin columns and washed. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 10 ml whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Stool DNA kit allows rapid and reliable isolation of high quality total DNA from fresh and frozen stool samples. Up to 200 mg of stool samples can be processed in less than 60 minutes. The system combines the reversible nucleic acid-binding properties of HiBind matrix with the speed and versatility of spin column technology to eliminate humic acid, polysaccharides, phenolic compounds, and enzyme inhibitors from stool samples. Purified DNA is suitable for PCR, restriction digestion, and hybridisation techniques.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® Plant RNA kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogeniser column for filtration and homogenisation of viscous plant cell lysate by centrifugation in combination with the HiBind® RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, northern blot analysis, differential display, and poly(A)+ RNA selection.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Bacterial DNA kit allows rapid and reliable isolation of high quality genomic DNA (gDNA) from a wide variety of bacterial species. Up to 0,5 ml gram positive or gram negative bacterial culture can be processed each time. The key to the system are the Mag-Bind® particles that avidly, but reversibly, bind DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed.

Supplier: OMEGA BIO-TEK

The E-Z 96® Plant DNA DS Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.® Plasmid Mini Kit I is available in either V-spin or Q-spin column format. V-spin column formats have an attached lid while Q-spin columns do not. Both versions can be used for centrifugation or vacuum protocols.

Supplier: OMEGA BIO-TEK

Pre-scripted solution for purification of high-quality DNA from blood, saliva, cultured cells, or fresh or frozen tissue.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription invitro, and other applications.

Supplier: Merck

Plasmid Miniprep 96 kit is a quick and easy-to-use kit that produces clean and reproducible DNA in half a time than traditional methods.

Supplier: OMEGA BIO-TEK

Isolate total RNA from cells or soft tissues using spin column.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Blood and Tissue DNA HDQ 96 Kit is designed for rapid and reliable isolation of high quality genomic DNA from 100 to 200 μl blood samples, buccal swabs, buffy coat and cultured cells. All heating steps that limit robotic applications have been removed to allow for faster processing. Mag-Bind® particles HDQ provide quick magnetic response time reducing overall processing time. Utilising paramagnetic particles provides high quality DNA that is suitable for direct use in most downstream applications, such as qPCR, PCR and Next Generation Sequencing. The Mag-Bind® HDQ Blood DNA and Tissue 96 Kit is compatible with many robotic liquid handlers and magnetic processors, including Thermo Scientific KingFisher® Flex.

Supplier: ENZO LIFE SCIENCES

EPIXTRACT® DNA isolation kit provides a simple method to isolate DNA from plasma, serum, and body fluids.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Plant DNA 96 plus kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of difficult to lyse and high polysaccharide plant species and tissues. The system utilises a CTAB-based lysis buffer without the need for a chloroform or other organic solvent steps. The proprietary wash buffer systems eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. The Mag-Bind® Plant DNA plus kit also provides greater flexibility than silica plate based systems as partial plates can be used and can be scaled up to a 24-well format with the appropriate magnetic stand. The streamlined protocol can be easily adapted on most robotic liquid handling platforms. Purified DNA is suitable for PCR, qPCR, restriction digestion, hybridisation applications, as well as more demanding applications, such as RAPD.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Food DNA Kit allows rapid and reliable isolation of high quality DNA from complex matrixes such as processed food, chocolate, cereals and meat.

Supplier: OMEGA BIO-TEK

MicroElute® Clean Up system, designed for rapid DNA clean up.

Supplier: Quantabio

Rapid extraction and purification of high-quality total RNA from cultured cells or tissue.

Supplier: OMEGA BIO-TEK

Isolate DNA from plant or fungal samples using spin columns.

Supplier: OMEGA BIO-TEK

Isolate host genomic, bacterial, fungal spore, and viral DNA and viral RNA from tissue, urine, serum, and fecal samples using magnetic beads.

Supplier: Corning

The AxyPrep™ Mag PCR Normalizer Kit utilizes a paramagnetic bead-based purification system for PCR clean up.

Supplier: Merck

The device utilises gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

Supplier: OMEGA BIO-TEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

Supplier: Copan

NAO® Basket for rapid and efficient DNA recovery. This is a semi-permeable system designed for releasing and concentrating human DNA from swab samples or other specimens during the extraction step. Lysis in the NAO® Basket: NAO® Basket retains the lysis buffer during the lysis step of the forensic sample.