5(6)-Carboxyfluorescein

Supplier: Abcam

Amine-reactive pH-sensitive fluorescent label. Covalently labels proteins and peptides. Tracks intracellular pH changes in vivo. 5-FAM and 6-FAM isomer mixture. 5(6)-CFDA and 5(6)-CFDA N-succinimidyl ester hydrolytic product.

Supplier: SIGMA ALDRICH MICROSCOPY

5(6)-Carboxyfluorescein 90% (dye content), Sigma-Aldrich®

Supplier: Abcam

Fluorescent, amine-reactive probe for bioconjugate synthesis. Labels bioconjugates on nucleophilic amines (N-terminus, lysine residues). Labeled bioconjugates resistant to hydrolysis. High stability and performance at physiological pH.

Supplier: Apollo Scientific

5(6)-Carboxyfluorescein

Supplier: Thermo Fisher Scientific

Appearance: Orange to brown Crystalline powder and/or chunks

Supplier: Thermo Fisher Scientific

5(6)-Carboxyfluorescein mixture of isomers 97%

Supplier: AAT Bioquest

Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations.

Supplier: Biotium

5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.