Loading Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

2X SDS-PAGE sample buffer without DTT or b-ME

Supplier: Rockland Immunochemicals

Sample buffer for protein electrophoresis.

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G-Biosciences TBE-Urea Sample Buffer

Supplier: G-Biosciences

Loading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands.

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6X GelRed prestain loading buffer with tracking dye

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

Sustainable Options Available

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Gel loading solution, 10X

Supplier: EDVOTEK

1 ml concentrate for 200 samples.

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Native sample loading buffer, 2X

Supplier: G-Biosciences

Loading Buffer, Native sample loading buffer (2X)

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TBE sample buffer (6X)

Supplier: Thermo Fisher Scientific

Loading Buffer, TBE Sample Buffer, 6X

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TRIS-glycine native sample buffer (4X), pH 8,6

Supplier: Thermo Fisher Scientific

Loading Buffer, TRIS-Glycine Native Sample Buffer (4X)

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Laemmli SDS sample buffer, reducing (6X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 6X

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Tricine-SDS sample buffer (2X), non-reducing

Supplier: Thermo Fisher Scientific

Loading Buffer, Tricine-SDS Sample Buffer (2X), non-reducing

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TBE urea sample buffer (2X)

Supplier: Thermo Fisher Scientific

Loading Buffer, TBE Urea Sample Buffer, 2X

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DNA gel loading buffer 6X

Supplier: LONZA

This buffer is a Ficoll based loading buffer containing Ficoll®, EDTA, xylene cyanol, and bromophenol blue. Ideal for separation of DNA fragments with improved resolution.

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Laemmli SDS sample buffer, non-reducing (6X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, non-reducing, 6X

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Laemmli SDS sample buffer, non-reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, non-reducing, 4X

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Laemmli SDS sample buffer with pyronin Y, reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, with PyroninY, 4X

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Loading buffer, SDS 4X, for SDS-PAGE analysis of proteins, Sigma-Aldrich®

Supplier: Merck Millipore (Novagen)

Loading Buffer, 4X SDS sample buffer

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LDS-sample buffer (4X), non-reducing

Supplier: Thermo Fisher Scientific

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.