You searched for: Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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RIPA buffer with Triton® X-100 (5X)

Supplier: Thermo Fisher Scientific

RIPA buffer with Triton® X-100 (5X), Thermo Fisher Scientific

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PIPES lysis buffer with NP-40 (2X)

Supplier: Thermo Fisher Scientific

PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

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Succinate, 0,2 M buffer solution, pH 5,0

Supplier: Thermo Fisher Scientific

Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific

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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

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GTE buffer

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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Triton® X-100 lysis buffer with glycerol (2X), pH 7,4

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific

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MNE buffer, pH 6,5

Supplier: Thermo Fisher Scientific

MNE buffer, pH 6.5, Thermo Fisher Scientific

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

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HEPES lysis buffer with NP-40 (2X)

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

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NP-40 lysis buffer, low salt

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, low salt, Thermo Fisher Scientific

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ACK Lysing Buffer (1x), TheraPEAK™

Supplier: LONZA

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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2-D Protein Extraction Buffer

Supplier: Cytiva

Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.