You searched for: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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Vector, pBR322, MP Biomedicals

Supplier: MP Biomedicals

pBR322 is a 4363 bp vector purified from Escherichia coli. It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).

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Expression vectors, cypridina Luc vector for luciferase assays, Pierce™

Supplier: Thermo Fisher Scientific

Cypridina Luc vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca.

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Polyadenylic acid (poly-A)

Supplier: Cytiva

Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.

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Expression vectors, red firefly Luc vector for Luciferase assays, Pierce™

Supplier: Thermo Fisher Scientific

Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.

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Gaussia Luc vector for luciferase assays, expression vectors, Pierce™

Supplier: Thermo Fisher Scientific

Gaussia Luc vectors contain a gene cloned from the copepod, Gaussia princeps. The gene encodes a naturally-secreted bioluminescent Gaussia luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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Vector, pUC 18, MP Biomedicals

Supplier: MP Biomedicals

pUC 18 is a small vector of approximately 2,7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.

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M13KO7 Helper Phage, Cytiva

Supplier: Cytiva

M13 phage for the GST expression system.

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Expression vectors, green renilla Luc vector for luciferase assays, Pierce™

Supplier: Thermo Fisher Scientific

Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis

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Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors

Supplier: Thermo Fisher Scientific

Gaussia Dura Luc vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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Expression vectors, pGEX

Supplier: Cytiva

The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.