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72 results for Vectors, Plasmids and Libraries

You searched for: Vectors, Plasmids and Libraries

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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Human Tagged ORF Clone Lentiviral Particle, PD-L1 (CD274)

Supplier: OriGene

Lenti ORF particles, CD274 (Myc-DDK tagged) - Human CD274 molecule (CD274), 200 µl, >10⁷ TU/ml.

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Human Tagged ORF Clone, CFTR (NM_000492)

Supplier: OriGene

This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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AAV helper-free system

Supplier: AGILENT

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

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Human Tagged ORF Clone, P Glycoprotein (ABCB1)

Supplier: OriGene

This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. ABCB1 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1).

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Human Tagged ORF Clone, GPCR MRGX2 (MRGPRX2)

Supplier: OriGene

MRGPRX2 (tGFP-tagged) - Human MAS-related GPR, member X2 (MRGPRX2). This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Untagged FBXL17 Clone

Supplier: OriGene

FBXL17 (untagged)-Human F-box and leucine-rich repeat protein 17 (FBXL17). The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10 µg of transfection-ready, dried plasmid DNA (reconstitute with 100 µl of water).

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Human shRNA plasmid kit, ADHFE1

Supplier: OriGene

Human shRNA plasmid kit, ADHFE1 is designed against multiple splice variants at this gene locus.

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Lambda gt11 vector kits

Lambda gt11 vector kits

Supplier: AGILENT

For use in the construction of cDNA libraries.

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ORF Clones, GABA B receptor 2 (GABBR2), human tagged, TrueORF Gold

Supplier: OriGene

GABA B receptor 2 (GABBR2), human tagged ORF clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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P2ry2 (NM_008773) Mouse Tagged ORF Clone

Supplier: OriGene

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use.

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Human cDNA Clone

Supplier: OriGene

KCNH2 (untagged) - human potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2), transcript variant 1

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AdEasy Adenoviral vector systems

Supplier: AGILENT

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

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pCMV-tag vectors

pCMV-tag vectors

Supplier: AGILENT

The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.

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Vectors, pBlueScript II

Supplier: AGILENT

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

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SureVector

SureVector

Supplier: AGILENT

Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.

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pGFP-C-shLenti shRNA Vector

Supplier: OriGene

pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.

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Human Untagged LRRC15 Clone

Supplier: OriGene

LRRC15 (untagged)-Human leucine rich repeat containing 15 (LRRC15), transcript variant 2.

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Transfection control plasmids, pVectOZ

Transfection control plasmids, pVectOZ

Supplier: OZ BIOSCIENCES

pVectOZ transfection plasmids are engineered in an optimised backbone and are ideal for all transfections.

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Cell-free protein expression vectors, T7, mammalian

Cell-free protein expression vectors, T7, mammalian

Supplier: Thermo Fisher Scientific

The T7 cell-free expression vectors (pT7CFE1) are cloning plasmids optimised for use with the Thermo Scientific™ 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation.

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Vector, pET-24d(+) DNA

Supplier: Merck Millipore (Novagen)

The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.

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Vector, pET-24(+) DNA

Supplier: Merck Millipore (Novagen)

pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His·Tag® sequence is available

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pET-24b(+) DNA, Novagen®

pET-24b(+) DNA, Novagen®

Supplier: Merck Millipore (Novagen)

The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

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pSTBlue-1 AccepTor™ vector kit

Supplier: Merck Millipore (Novagen)

The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant Taq polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues in vivo following transformation.

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pET-14b DNA, Novagen®

Supplier: Merck Millipore (Novagen)

The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.

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pET-30a(+) DNA, Novagen®

Supplier: Merck Millipore (Novagen)

The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.

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Slc7a11 (NM_011990) mouse tagged ORF clone

Supplier: OriGene

Slc7a11 (GFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter y+ system) member 11 (Slc7a11), (10 µg).

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Expression vectors, pET-41 DNA, Novagen®

Expression vectors, pET-41 DNA, Novagen®

Supplier: Merck Millipore (Novagen)

pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

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