Cooked Meat Medium and the enriched medium are used for the cultivation of anaerobes, especially pathogenic clostridia.

• Muscle protein provides amino acids and other nutrients
• Muscle provides reducing substances to permit growth of strict anaerobes
• Growth is indicated by turbidity and presence of gas bubbles

Cooked Meat Medium provides a favorable environment for the growth of anaerobes. The sulfhydryl groups, which exert the reducing effect, are more available in denatured protein; therefore, the meat particles are cooked for use in the medium.