Cell Culture Buffers
Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.
VWR® TRIS Buffer, 1 M Solution, pH 8.0
Supplier: VWR
Tris (hydroxymethyl) aminomethane
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VWR® Phosphate Buffered Saline (PBS), 10X, Ultra Pure Grade
Supplier: VWR
1X Solution Composition: 137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer.
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VWR® Ultra Pure Grade Phosphate Buffered Saline
Supplier: VWR
1X Solution Composition:137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer
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VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade
Supplier: VWR
1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer.Does not contain magnesium or calcium.
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VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade
Supplier: VWR
Preparation of PBS using 10 L and 50 L Format:
Dissolve 9.88 g of PBS powder in 1 L of water to prepare a 1X PBS solution containing 137 mM sodium chloride, 2.7 mM potassium chloride and 9.8 mM phosphate buffer.
The 10 L and 50 L pack sizes contain sufficient powder to prepare 10 L and 50 L of 1X PBS, respectively.
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VWR® Phosphate Buffered Saline, Ultra Pure Grade
Supplier: VWR
1X Solution Composition: 137 mM NaCl, 2.7 mM KCl, 9.8 mM Phosphate buffer
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HyClone™ Dulbecco's Phosphate Buffered Saline Solution, HyClone products (Cytiva)
Supplier: Cytiva
Balanced salt solution used for multiple cell culture applications, (liquid form).
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STERI-Buffer®, Veltek Associates
Supplier: VELTEK ASSOCIATES, INC. MX
Developed for use as a buffer solution and diluent in lab testing such a bioburden testing, pharmaceutical microbial limits testing, and AET testing and as a rinsing agent."
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Blood Bank Saline Solutions, Azer Scientific
Supplier: AZER SCIENTIFIC INC
Blood bank saline is pH-controlled for a pH between 6.5 and 7.0. Buffered saline is tightly controlled to a pH range of 7.0 to 7.2 and is specially designed to enhance weak agglutination reactions in blood bank procedures. Both saline and buffered saline remain stable without preservatives. All of the solutions meet NCCLS specifications for standard isotonic sodium chloride solution for use in immunohematologic testing. Containers feature a convenient dispensing spigot.
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