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Triton® X-100 (Polyethylene glycol tert-octylphenyl ether) for biochemistry

Triton® X-100 (Polyethylene glycol tert-octylphenyl ether) for biochemistry

Supplier: VWR

Non-ionic detergent suited for solubilizing membrane proteins without altering biological activity.

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Cetrimonium bromide

Cetrimonium bromide

Supplier: VWR

A cationic positively-charged quaternary ammonium compounds. Utilized for applications ranging from solubilizing membrane proteins in non-denaturing conditions to creating denaturing conditions during gel electrophoresis.

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Eosin Y (yellowish) for histology

Eosin Y (yellowish) for histology

Supplier: VWR

Counterstain to hematoxylin. Used for staining exfoliative cytology.

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VWR® Casamino Acids

VWR® Casamino Acids

Supplier: VWR

VWR offers high purity protein digests used for media supplements.

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Toluidine blue O, powder

Toluidine blue O, powder

Supplier: VWR

A blue counterstain often used for metachromatic staining of mast cells, cartilage, and acid mucins. Black to dark green fine powder

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VWR® pH Standards

VWR® pH Standards

Supplier: VWR

VWR®

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Chloroform : iso-Amyl alcohol (24:1 v:v)

Chloroform : iso-Amyl alcohol (24:1 v:v)

Supplier: VWR

Chloroform : iso-Amyl alcohol (24:1 v:v)

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HEPES 2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulphonic acid for biochemistry

HEPES 2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulphonic acid for biochemistry

Supplier: VWR

One of the best general-purpose buffers for biological research. Excellent for tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, and CO2 fixation.

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Sodium hydroxide

Sodium hydroxide

Supplier: VWR

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VWR® Rapid Transfer Buffer, 10X

VWR® Rapid Transfer Buffer, 10X

Supplier: VWR

Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications

    
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Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) for biochemistry

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) for biochemistry

Supplier: VWR

Reagent for stabilization of buffer formulations and electrophoresis of biological molecules.

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VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade

VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade

Supplier: VWR

Preparation of PBS using 10 L and 50 L Format:
Dissolve 9.88 g of PBS powder in 1 L of water to prepare a 1X PBS solution containing 137 mM sodium chloride, 2.7 mM potassium chloride and 9.8 mM phosphate buffer.
The 10 L and 50 L pack sizes contain sufficient powder to prepare 10 L and 50 L of 1X PBS, respectively.

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Urea

Urea

Supplier: VWR

Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the acti

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Orthophosphoric acid ( 85%) 85% ACS

Orthophosphoric acid ( 85%) 85% ACS

Supplier: VWR

Orthophosphoric acid ( 85%) 85% ACS

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Sodium sulphate ACS

Sodium sulphate ACS

Supplier: VWR

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Potassium iodide ACS

Potassium iodide ACS

Supplier: VWR

Potassium iodide ACS

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Formamide

Formamide

Supplier: VWR

Formamide

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Sodium molybdate(VI) dihydrate ACS
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Dithiothreitol (DTT, Cleland's reagent) for biochemistry

Dithiothreitol (DTT, Cleland's reagent) for biochemistry

Supplier: VWR

Dithiothreitol (DTT, Cleland's reagent) for biochemistry

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VWR® Bovine Ribonuclease A (from Pancreas)

VWR® Bovine Ribonuclease A (from Pancreas)

Supplier: VWR

Ribonuclease A is an endoribonuclease that efficiently hydrolyzes RNA contaminants in DNA preparations from tissue or bacterial cell cultures. RNase A is used for a variety of molecular biology applications, most commonly for the hydrolysis of RNA that contaminates DNA preparations. This enzyme is used during plasmid purification without nicking or degrading plasmid.

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Dithiothreitol (DTT, Cleland's reagent)

Dithiothreitol (DTT, Cleland's reagent)

Supplier: VWR

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

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3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

Supplier: VWR

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Ultrapure

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Ethanol absolute (denatured)

Ethanol absolute (denatured)

Supplier: VWR

Denatured with 5% methanol

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VWR® Peptone

VWR® Peptone

Supplier: VWR

High purity protein digests used for media supplements.

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Dimethyl sulphoxide

Dimethyl sulphoxide

Supplier: VWR

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Glycogen 20 mg/ml

Glycogen 20 mg/ml

Supplier: VWR

AMRESCO’s molecular biology grade glycogen is an inert carrier that increases the recovery of nucleic acids after alcohol precipitation. Insoluble in ethanol or isoproanol, glycogen traps the nucleic acids during precipitation and forms a visible pellet upon centrifugation. As an inert co-precipitant, glycogen efficiently recovers from dilute solutions DNA or RNA molecules ranging in size from small oligonucleotides to megabase molecules

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VWR® MOPS Buffer Solution 10X, Biotechnology Grade

VWR® MOPS Buffer Solution 10X, Biotechnology Grade

Supplier: VWR

Optimized for RNA Agarose Electrophoresis

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Ammonium sulphate

Ammonium sulphate

Supplier: VWR

May be used for the precipitation or fractionation of proteins or for purification of antibodies. Useful for crystallographic analysis of nucleic acids and proteins.

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CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate)

CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate)

Supplier: VWR

Zwitterionic, both positively and negatively charged. Utilized for applications ranging from solubilizing membrane proteins in non-denaturing conditions to creating denaturing conditions during gel electrophoresis.

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