301 Results for: "VWR"
Sodium thiosulphate (0.1 - 1 mol/l; 0.1 - 1 N) in aqueous solution 0.1 N analytical reagent
Supplier: VWR
Suitable as a titrant. Made with deionized water.
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Ammonium sulphate
Supplier: VWR
May be used for the precipitation or fractionation of proteins or for purification of antibodies. Useful for crystallographic analysis of nucleic acids and proteins.
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Dithiothreitol (DTT, Cleland's reagent)
Supplier: VWR
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
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VWR® Bovine Ribonuclease A (from Pancreas)
Supplier: VWR
Ribonuclease A is an endoribonuclease that efficiently hydrolyzes RNA contaminants in DNA preparations from tissue or bacterial cell cultures. RNase A is used for a variety of molecular biology applications, most commonly for the hydrolysis of RNA that contaminates DNA preparations. This enzyme is used during plasmid purification without nicking or degrading plasmid.
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Dithiothreitol (DTT, Cleland's reagent) for biochemistry
Supplier: VWR
Dithiothreitol (DTT, Cleland's reagent) for biochemistry
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Glycogen 20 mg/ml
Supplier: VWR
AMRESCO’s molecular biology grade glycogen is an inert carrier that increases the recovery of nucleic acids after alcohol precipitation. Insoluble in ethanol or isoproanol, glycogen traps the nucleic acids during precipitation and forms a visible pellet upon centrifugation. As an inert co-precipitant, glycogen efficiently recovers from dilute solutions DNA or RNA molecules ranging in size from small oligonucleotides to megabase molecules
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3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
Supplier: VWR
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Ultrapure
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VWR® Peptone
Supplier: VWR
High purity protein digests used for media supplements.
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VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade
Supplier: VWR
Preparation of PBS using 10 L and 50 L Format:
Dissolve 9.88 g of PBS powder in 1 L of water to prepare a 1X PBS solution containing 137 mM sodium chloride, 2.7 mM potassium chloride and 9.8 mM phosphate buffer.
The 10 L and 50 L pack sizes contain sufficient powder to prepare 10 L and 50 L of 1X PBS, respectively.
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Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) for biochemistry
Supplier: VWR
Reagent for stabilization of buffer formulations and electrophoresis of biological molecules.
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Orthophosphoric acid ( 85%) 85% ACS
Supplier: VWR
Orthophosphoric acid ( 85%) 85% ACS
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VWR® TAQ DNA Polymerase
Supplier: VWR
Taq DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity.
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VWR® TRIS-Borate-EDTA Buffer, 5X Concentrate, Ultra Pure Grade
Supplier: VWR
TBE is optimized for DNA electrophoresis techniques using both acrylamide and agarose
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VWR® Rapid Transfer Buffer, 10X
Supplier: VWR
Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications
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HEPES 2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulphonic acid for biochemistry
Supplier: VWR
One of the best general-purpose buffers for biological research. Excellent for tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, and CO2 fixation.
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VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade
Supplier: VWR
1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer.Does not contain magnesium or calcium.
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CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate)
Supplier: VWR
Zwitterionic, both positively and negatively charged. Utilized for applications ranging from solubilizing membrane proteins in non-denaturing conditions to creating denaturing conditions during gel electrophoresis.