301 Results for: "VWR"
Ammonium sulphate
Supplier: VWR
May be used for the precipitation or fractionation of proteins or for purification of antibodies. Useful for crystallographic analysis of nucleic acids and proteins.
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Sodium D-gluconate 98-102%
Supplier: VWR
Sodium gluconate 98-102%, crystals, high purity
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Dithiothreitol (DTT, Cleland's reagent)
Supplier: VWR
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
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HEPES 2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulphonic acid for biochemistry
Supplier: VWR
One of the best general-purpose buffers for biological research. Excellent for tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, and CO2 fixation.
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Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) for biochemistry
Supplier: VWR
Reagent for stabilization of buffer formulations and electrophoresis of biological molecules.
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VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade
Supplier: VWR
Preparation of PBS using 10 L and 50 L Format:
Dissolve 9.88 g of PBS powder in 1 L of water to prepare a 1X PBS solution containing 137 mM sodium chloride, 2.7 mM potassium chloride and 9.8 mM phosphate buffer.
The 10 L and 50 L pack sizes contain sufficient powder to prepare 10 L and 50 L of 1X PBS, respectively.
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3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS), Ultrapure
Supplier: VWR
Zwitterionic, both positively and negatively charged. Utilized for applications ranging from solubilizing membrane proteins in non-denaturing conditions to creating denaturing conditions during gel electrophoresis.
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VWR® MOPS Buffer Solution 10X, Biotechnology Grade
Supplier: VWR
Optimized for RNA Agarose Electrophoresis
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VWR® Rapid Transfer Buffer, 10X
Supplier: VWR
Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications
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Guanidinium chloride for biochemistry
Supplier: VWR
Suited for purification of nucleic acids or polypeptides from cell sources and inhibition of both RNase and protease activity.
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VWR® TRIS-Borate-EDTA Buffer, 5X Concentrate, Ultra Pure Grade
Supplier: VWR
TBE is optimized for DNA electrophoresis techniques using both acrylamide and agarose
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Urea 99.0-100.5% ACS
Supplier: VWR
Urea 99.0-100.5%, crystallised ACS, VWR Chemicals BDH®
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Chloroform : iso-Amyl alcohol (24:1 v:v)
Supplier: VWR
Chloroform : iso-Amyl alcohol (24:1 v:v)
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Dimethyl sulphoxide
Supplier: VWR
Dimethyl sulphoxide ≥99.9%, Ultra Pure Grade
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VWR® TAQ DNA Polymerase
Supplier: VWR
Taq DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity.
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VWR® Peptone
Supplier: VWR
High purity protein digests used for media supplements.
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VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade
Supplier: VWR
1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer.Does not contain magnesium or calcium.
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2-Mercaptoethanol ≥99%
Supplier: VWR
2-Mercaptoethanol ≥99% for biotechnology
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Sodium molybdate(VI) dihydrate ACS
Supplier: VWR
Sodium molybdate(VI) dihydrate ACS
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Iron (II) sulphate heptahydrate
Supplier: VWR
Iron(II) sulphate heptahydrate ≥99% ACS
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Urea
Supplier: VWR
Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the acti
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Sodium thiosulphate 0.10 N in aqueous solution analytical reagent, VWR Chemicals BDH®
Supplier: VWR
Suitable as a titrant. Made with deionized water.
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Glycogen 20 mg/ml
Supplier: VWR
AMRESCO’s molecular biology grade glycogen is an inert carrier that increases the recovery of nucleic acids after alcohol precipitation. Insoluble in ethanol or isoproanol, glycogen traps the nucleic acids during precipitation and forms a visible pellet upon centrifugation. As an inert co-precipitant, glycogen efficiently recovers from dilute solutions DNA or RNA molecules ranging in size from small oligonucleotides to megabase molecules