Advanced one-tube qPCR supermix optimized to support highly multiplexed DNA amplifications in miniaturized reaction volumes and withstand a broad spectrum of PCR inhibitors.

• Tough Tested - Overcomes common inhibitors including polysacharides, heme/hemoglobin, humic acid, melanin
• Flexible - Use fast or standard qPCR cycling conditions
• Broad dynamic range - Reliable data from your precious samples every time

PerfeCTa® MultiPlex qPCR ToughMix is a 5X concentrated, ready-to-use reaction cocktail for real-time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta MultiPlex qPCR ToughMix has been optimized to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR that can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa MultiPlex qPCR ToughMix results in multiplexed qPCR with dynamic range and sensitivity that are comparable to single-plex qPCR probe assays without the need for limiting or variable primer concentrations.

A key component of PerfeCta MultiPlex qPCR ToughMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeCta Multiplex qPCR ToughMix 5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, and stabilizers.

PerfeCta® Multiplex qPCR ToughMix ROX™ 5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye and stabilizers.

PerfeCta® Multiplex qPCR ToughMix® Low ROX™ 5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye and stabilizers.

PerfeCta Multiplex qPCR ToughMix is stable for 2 years when stored in a constant temperature freezer at 20ºC, protected from light. For convenience, it may be stored unfrozen at +2 to +8ºC for up to 6 months. Repeated freezing and thawing of the ToughMix will not affect product performance.