PerfeCTa qPCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues

• ToughMix formulation neutralize PCR inhibitors to ensure reliable assay performance with a variety of starting materials
• Exceptional reagent stability (uninhibited after 20X freeze-thaw or 30 days at 22 °C) and anti-foam technology for efficient vortex mixing
• Easy-to-use 2X concentrated mastermixes with AccuVue™ plate loading dye and optimized passive reference dye

A key component of PerfeCTa qPCR ToughMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeCTa qPCR ToughMix delivers exceptional performance with either fast or conventional PCR cycling protocols.

PerfeCTa qPCR ToughMix is compatible with many Real-Time PCR Instruments, such as the Roche LightCycler™ 480, Qiagen/Corbett Rotor-Gene® Q, Qiagen/Corbett Rotor-Gene® 6000, Qiagen/Corbett Rotor-Gene® 3000, Eppendorf Mastercycler® ep realplex, Eppendorf Mastercycler® ep realplex2 s, Cepheid SmartCycler®, Bio-Rad CFX96™, Bio-Rad CFX384™, Bio-Rad/MJ Opticon®, Bio-Rad/MJ Opticon 2, Bio-Rad/MJ Chromo4™, Bio-Rad MiniOpticon™, Illumina Eco qPCR, Thermo Scientific PikoReal Cycler, BioRad iCycler iQ™, BioRad MyiQ™ and BioRad iQ™5.

PerfeCTa qPCR ToughMix is stable for 2 years when stored in a constant temperature freezer at –20°C. For convenience, it may be stored unfrozen at 2 to 8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing does not affect PCR performance.

It is a versatile and robust real-time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa qPCR ToughMix contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.