Tissue culture buffers provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure present. Used predominately in biomolecular research, the dry and liquid substances have a balanced inorganic salt composition. Lower concentration options permit equilibration with air in a closed system, while higher salt concentration options keep gaseous phases in equilibrium. Cell growth is optimal when plated in a nutritional medium that has been buffered.

VWR® TRIS Buffer, 1 M Solution, pH 8.0

Supplier: VWR

Tris (hydroxymethyl) aminomethane

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VWR® Ultra Pure Grade Phosphate Buffered Saline

Supplier: VWR

1X Solution Composition:137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer

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VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade

Supplier: VWR

1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer.Does not contain magnesium or calcium.

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VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade

Supplier: VWR

Preparation of PBS using 10 L and 50 L Format:
Dissolve 9.88 g of PBS powder in 1 L of water to prepare a 1X PBS solution containing 137 mM sodium chloride, 2.7 mM potassium chloride and 9.8 mM phosphate buffer.
The 10 L and 50 L pack sizes contain sufficient powder to prepare 10 L and 50 L of 1X PBS, respectively.

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VWR® Phosphate Buffered Saline, Ultra Pure Grade

Supplier: VWR

1X Solution Composition: 137 mM NaCl, 2.7 mM KCl, 9.8 mM Phosphate buffer

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VWR® Phosphate Buffered Saline (PBS), 10X, Ultra Pure Grade

Supplier: VWR

1X Solution Composition: 137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer.

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HyClone™ Dulbecco's Phosphate Buffered Saline Solution, HyClone products (Cytiva)

Supplier: Cytiva

Balanced salt solution used for multiple cell culture applications, (liquid form).

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STERI-Buffer®, Veltek Associates

Supplier: VELTEK ASSOCIATES, INC. MX

Developed for use as a buffer solution and diluent in lab testing such a bioburden testing, pharmaceutical microbial limits testing, and AET testing and as a rinsing agent."