주문 입력
Korea, Republic of
Orders LinkContactUsLinkComponent
 

TEMPase Hot start DNA 중합효소

TEMPase Hot start DNA 중합효소
공급사:  VWR Chemicals
Certificates
TEMPase Hot start DNA 중합효소

7 Options Available Below

Product Details & Documents

VWR® TEMPase Hot Start DNA 중합효소는 매우 안정적인 중합효소로, 표준 DNA 중합효소에 비해 더 높은 특이성, 민감도 및 더 높은 산출량을 특징으로 하는 매우 안정적인 중합효소입니다. 이러한 특징들로 인해 낮은 농도의 목표물을 탐지하는 데 매우 적합합니다. 다른 용도에는 스크리닝, GC-rich 시퀀스의 증폭, multiplex PCR, 직접적인 PCR 및 qPCR이 포함됩니다. 글리세롤이 없는 TEMPase Hot Start DNA 중합효소는 자동화 및 동결 건조에도 사용할 수 있습니다.

GC-Rich 템플릿 키트는 어려운 GC-rich 시퀀스를 위해 특별히 설계되었습니다. TEMPase와 결합된 GC 버퍼 I 및 II는 우수한 증폭을 촉진합니다. 키트는 GC-TEMPase 2X Master Mix 중 하나를 사용하기 전 사전 테스트를 위해 설계되었습니다.

Delivery information: VWR® TEMPase DNA 중합효소는 일반적으로 Key Buffer와 Combination Buffer의 두 가지 다른 버퍼를 포함하며, 이들은 각기 다른 PCR 요구 사항에 적합합니다. Key Buffer(NH⁴⁺)는 우수한 증폭 신호(높은 산출량)를 제공하고 대부분의 primer-template 시스템에서 Mg²⁺ 농도 또는 어닐링 온도의 최적화 필요성을 최소화합니다 . 조합 버퍼는 K + 와 NH⁴⁺ 의 혼합물입니다. 프라이머 어닐링 온도 및 Mg²⁺ 최적화에 대한 높은 특이성과 우수한 제품 산출량 및 높은 내성을 갖고 있는데, 이는 암모늄-칼륨의 균형 잡힌 제형때문입니다. 각 버퍼에는 15 mM MgCl₂ (최종 부피 1,5 mM)가 포함되어 있습니다. 간편한 최적화를 위한 추가 MgCl₂는 별도의 바이알에 포함되어 있습니다.

Specifications for Product Family

사양

사양

  • Catalog No:
  • 733-1331
  • 733-1333
  • 733-1838
  • 733-2552
  • 733-2553
  • 733-2556
  • 733-2559
  • Description:
  • TEMPase HotStart DNA Polymerase 5U/µl 500 units
  • TEMPase HotStart DNA Polymerase 5U/µl 2500 units
  • TEMPase HotStart DNA Polymerase 5U/µl 10.000 units
  • TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 500 units
  • TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 2500 units
  • TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 2500 units
  • TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 2500 units
  • Format:
  • Enzyme with/without buffer
  • Enzyme with/without buffer
  • Enzyme with/without buffer
  • Enzyme with/without buffer
  • Enzyme with/without buffer
  • Enzyme with/without buffer
  • Enzyme with/without buffer
  • Features:
  • Glycerol free
  • Glycerol free
  • Glycerol free
  • Glycerol free
  • Hot start:
  • Chemically modified
  • Chemically modified
  • Chemically modified
  • Chemically modified
  • Chemically modified
  • Chemically modified
  • Chemically modified
  • Concentration:
  • 5 U/µl
  • 5 U/µl
  • 5 U/µl
  • 5 U/µl
  • 5 U/µl
  • 5 U/µl
  • 5 U/µl
  • Units:
  • 500
  • 2500
  • 10000
  • 500
  • 2500
  • 2500
  • 2500
  • Size:
  • 1 x 0.1 ml + 3 x 1.5 ml
  • 5 x 0.1 ml + 15 x 1.5 ml
  • 3 x 0.667 ml + 18 x 5 ml
  • 1 x 0.1 ml + 1 x 1.5 ml
  • 5 x 0.1 ml + 5 x 1.5 ml
  • 5 x 0.1 ml + 10 x 1.5 ml
  • 5 x 0.1 ml + 10 x 1.5 ml
  • Buffer composition:
  • 10x Key Buffer, 10x Combination Buffer and 25 mM MgCl2
  • 10x Key Buffer, 10x Combination Buffer and 25 mM MgCl2
  • 10x Key Buffer, 10x Combination Buffer and 25 mM MgCl2
  • 25 mM MgCl2
  • 25 mM MgCl2
  • 10x Key Buffer and 25 mM MgCl2
  • 10x Combination Buffer and 25 mM MgCl2
  • High fidelity:
  • No
  • No
  • No
  • No
  • No
  • No
  • No
  • Amplicon size:
  • ≤ 5kb
  • ≤ 5kb
  • ≤ 5kb
  • ≤ 5kb
  • ≤ 5kb
  • ≤ 5kb
  • ≤ 5kb
  • Processivity:
  • Standard
  • Standard
  • Standard
  • Standard
  • Standard
  • Standard
  • Standard
  • Exonuclease activity:
  • 5’→3’
  • 5’→3’
  • 5’→3’
  • 5’→3’
  • 5’→3’
  • 5’→3’
  • 5’→3’
  • Product overhang:
  • 3'A
  • 3'A
  • 3'A
  • 3'A
  • 3'A
  • 3'A
  • 3'A
  • Storage conditions:
  • – 20 °C (long term), + 4 °C (6 months)
  • – 20 °C (long term), + 4 °C (6 months)
  • – 20 °C (long term), + 4 °C (6 months)
  • – 20 °C (long term), + 4 °C (6 months)
  • – 20 °C (long term), + 4 °C (6 months)
  • – 20 °C (long term), + 4 °C (6 months)
  • – 20 °C (long term), + 4 °C (6 months)
  • Application:
  • Multiplex
  • Multiplex
  • Multiplex
  • Freeze-drying|Standard amplification
  • Freeze-drying|Standard amplification
  • Freeze-drying|Standard amplification
  • Freeze-drying|Standard amplification

사양

Product Family Options

제품 정보

  • Pk
    재고 여부
    Price
  • 733-1331
    TEMPase Hot Start DNA Polymerase, 5 U/µl, with 10X Key Buffer, 10X Combination Buffer and MgCl2
    TEMPase Hot Start DNA Polymerase, 5 U/µl, with 10X Key Buffer, 10X Combination Buffer and MgCl2
    500 EU
    Log in 재고 확인
    Log in 재고 확인
    1 * 500 EU
    1 * 500 EU - ₩543,943
    ₩543,943
    1 * 500 EU
       ₩543,943

    사양:
    More
    Specifications
    Cat. No.
    733-1331
    Description
    TEMPase HotStart DNA Polymerase 5U/µl 500 units
    Format
    Enzyme with/without buffer
    Features
    Hot start
    Chemically modified
    Concentration
    5 U/µl
    Units
    500
    Size
    1 x 0.1 ml + 3 x 1.5 ml
    Buffer composition
    10x Key Buffer, 10x Combination Buffer and 25 mM MgCl2
    High fidelity
    No
    Amplicon size
    ≤ 5kb
    Processivity
    Standard
    Exonuclease activity
    5’→3’
    Product overhang
    3'A
    Storage conditions
    – 20 °C (long term), + 4 °C (6 months)
    Application
    Multiplex
  • 733-1333
    TEMPase Hot Start DNA Polymerase, 5 U/µl, with 10X Key Buffer, 10X Combination Buffer and MgCl2
    TEMPase Hot Start DNA Polymerase, 5 U/µl, with 10X Key Buffer, 10X Combination Buffer and MgCl2
    2.500 EU
    Log in 재고 확인
    Log in 재고 확인
    1 * 2.500 EU
    1 * 2.500 EU - ₩2,189,507
    ₩2,189,507
    1 * 2.500 EU
       ₩2,189,507

    사양:
    More
    Specifications
    Cat. No.
    733-1333
    Description
    TEMPase HotStart DNA Polymerase 5U/µl 2500 units
    Format
    Enzyme with/without buffer
    Features
    Hot start
    Chemically modified
    Concentration
    5 U/µl
    Units
    2500
    Size
    5 x 0.1 ml + 15 x 1.5 ml
    Buffer composition
    10x Key Buffer, 10x Combination Buffer and 25 mM MgCl2
    High fidelity
    No
    Amplicon size
    ≤ 5kb
    Processivity
    Standard
    Exonuclease activity
    5’→3’
    Product overhang
    3'A
    Storage conditions
    – 20 °C (long term), + 4 °C (6 months)
    Application
    Multiplex
  • 733-1838
    TEMPase Hot Start DNA Polymerase, 5 U/µl, with 10X Key Buffer, 10X Combination Buffer and MgCl2
    TEMPase Hot Start DNA Polymerase, 5 U/µl, with 10X Key Buffer, 10X Combination Buffer and MgCl2
    10.000 EU
    Log in 재고 확인
    Log in 재고 확인
    1 * 10.000 EU
    1 * 10.000 EU - ₩6,767,207
    ₩6,767,207
    1 * 10.000 EU
       ₩6,767,207

    사양:
    More
    Specifications
    Cat. No.
    733-1838
    Description
    TEMPase HotStart DNA Polymerase 5U/µl 10.000 units
    Format
    Enzyme with/without buffer
    Features
    Hot start
    Chemically modified
    Concentration
    5 U/µl
    Units
    10000
    Size
    3 x 0.667 ml + 18 x 5 ml
    Buffer composition
    10x Key Buffer, 10x Combination Buffer and 25 mM MgCl2
    High fidelity
    No
    Amplicon size
    ≤ 5kb
    Processivity
    Standard
    Exonuclease activity
    5’→3’
    Product overhang
    3'A
    Storage conditions
    – 20 °C (long term), + 4 °C (6 months)
    Application
    Multiplex
  • 733-2552
    TEMPase Hot Start DNA Polymerase, glycerol-free, 5 U/µl, without buffers
    TEMPase Hot Start DNA Polymerase, glycerol-free, 5 U/µl, without buffers
    500 Tests
    Log in 재고 확인
    Log in 재고 확인
    1 * 500 Tests
    1 * 500 Tests - ₩477,686
    ₩477,686
    1 * 500 Tests
       ₩477,686

    사양:
    More
    Specifications
    Cat. No.
    733-2552
    Description
    TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 500 units
    Format
    Enzyme with/without buffer
    Features
    Glycerol free
    Hot start
    Chemically modified
    Concentration
    5 U/µl
    Units
    500
    Size
    1 x 0.1 ml + 1 x 1.5 ml
    Buffer composition
    25 mM MgCl2
    High fidelity
    No
    Amplicon size
    ≤ 5kb
    Processivity
    Standard
    Exonuclease activity
    5’→3’
    Product overhang
    3'A
    Storage conditions
    – 20 °C (long term), + 4 °C (6 months)
    Application
    Freeze-drying|Standard amplification
  • 733-2553
    TEMPase Hot Start DNA Polymerase, glycerol-free, 5 U/µl, without buffers
    TEMPase Hot Start DNA Polymerase, glycerol-free, 5 U/µl, without buffers
    2.500 Tests
    Log in 재고 확인
    Log in 재고 확인
    1 * 2.500 Tests
    1 * 2.500 Tests - ₩2,043,457
    ₩2,043,457
    1 * 2.500 Tests
       ₩2,043,457

    사양:
    More
    Specifications
    Cat. No.
    733-2553
    Description
    TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 2500 units
    Format
    Enzyme with/without buffer
    Features
    Glycerol free
    Hot start
    Chemically modified
    Concentration
    5 U/µl
    Units
    2500
    Size
    5 x 0.1 ml + 5 x 1.5 ml
    Buffer composition
    25 mM MgCl2
    High fidelity
    No
    Amplicon size
    ≤ 5kb
    Processivity
    Standard
    Exonuclease activity
    5’→3’
    Product overhang
    3'A
    Storage conditions
    – 20 °C (long term), + 4 °C (6 months)
    Application
    Freeze-drying|Standard amplification
  • 733-2556
    TEMPase Hot Start DNA Polymerase, glycerol-free, 5 U/µl, with 10X Key Buffer and MgCl2
    TEMPase Hot Start DNA Polymerase, glycerol-free, 5 U/µl, with 10X Key Buffer and MgCl2
    2.500 Tests
    Log in 재고 확인
    Log in 재고 확인
    1 * 2.500 Tests
    1 * 2.500 Tests - ₩2,123,029
    ₩2,123,029
    1 * 2.500 Tests
       ₩2,123,029

    사양:
    More
    Specifications
    Cat. No.
    733-2556
    Description
    TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 2500 units
    Format
    Enzyme with/without buffer
    Features
    Glycerol free
    Hot start
    Chemically modified
    Concentration
    5 U/µl
    Units
    2500
    Size
    5 x 0.1 ml + 10 x 1.5 ml
    Buffer composition
    10x Key Buffer and 25 mM MgCl2
    High fidelity
    No
    Amplicon size
    ≤ 5kb
    Processivity
    Standard
    Exonuclease activity
    5’→3’
    Product overhang
    3'A
    Storage conditions
    – 20 °C (long term), + 4 °C (6 months)
    Application
    Freeze-drying|Standard amplification
  • 733-2559
    TEMPase Hot Start DNA Polymerase, glycerol-free, 5 U/µl, with 10X Combination Buffer and MgCl2
    TEMPase Hot Start DNA Polymerase, glycerol-free, 5 U/µl, with 10X Combination Buffer and MgCl2
    2.500 Tests
    Log in 재고 확인
    Log in 재고 확인
    1 * 2.500 Tests
    1 * 2.500 Tests - ₩2,123,029
    ₩2,123,029
    1 * 2.500 Tests
       ₩2,123,029

    사양:
    More
    Specifications
    Cat. No.
    733-2559
    Description
    TEMPase HotStart DNA Polymerase Glycereol Free 5U/µl 2500 units
    Format
    Enzyme with/without buffer
    Features
    Glycerol free
    Hot start
    Chemically modified
    Concentration
    5 U/µl
    Units
    2500
    Size
    5 x 0.1 ml + 10 x 1.5 ml
    Buffer composition
    10x Combination Buffer and 25 mM MgCl2
    High fidelity
    No
    Amplicon size
    ≤ 5kb
    Processivity
    Standard
    Exonuclease activity
    5’→3’
    Product overhang
    3'A
    Storage conditions
    – 20 °C (long term), + 4 °C (6 months)
    Application
    Freeze-drying|Standard amplification
Recommendations

Recommendations will be personalized based on your shopping preferences only if you have given your consent by enabling "Enhance my Shopping Experience" on the "Personal Info page".

Otherwise, you will receive generic recommendations.