53 Results for: "Electrophoresis Buffers"

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.

Supplier: VWR Chemicals

PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.

Supplier: VWR Collection

Based on the Maxi M model, the Maxi M 'Revolution' offers a built-in buffer circulation system, powered by the system’s electrodes. At the cathode, hydrogen bubbles are produced and pass up a diagonally positioned pipe connected to the two sides of the tank to produce a gentle and continual circulation of the buffer. The system nulls any pH and ionic gradient – without the fuss of mechanical pumps. The system offers 6 comb positions enabling the gel to be partitioned into 2, 3 or 4 areas. In addition, the system can accommodate any of the 23 standard and microtitre comb designs described for the Maxi M model.

Supplier: VWR Chemicals

Convenient liquid concentrate that produces 10 litres of 1X buffer for blotting and ELISA applications.

Supplier: VWR Chemicals

EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.

Supplier: VWR Chemicals

RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.

Supplier: VWR Chemicals

EZ-Vision DNA dye as loading buffer provides a non mutagenic, environmentally friendly alternative to ethidium bromide for instantaneous fluorescent DNA band visualisation.

Supplier: VWR Chemicals

VWR® DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

Supplier: VWR Chemicals

VWR® Red Taq DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

Supplier: VWR Chemicals

This loading dye base is used for custom loading buffer preparation. It is a concentrated solution containing patent blue, bromophenol blue and amaranth tracking dyes, which migrate at approximately 4 000, 400 and 10 bp, on a 1% agarose gel, respectively.

Supplier: VWR Chemicals

These DNA loading dyes are used to load DNA samples to agarose or SDS DNA gels for gel electrophoresis.

Supplier: VWR Chemicals

A convenient pre-mixed solution that is easily diluted for buffer preparation under denaturing conditions.Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions.

Supplier: VWR Chemicals

A zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA.

Supplier: VWR Collection

For 16×14 cm gels, the Twin M is a versatile system for SDS PAGE applications, in addition to non denaturing electrophoresis of proteins, SSCPs and dinucleotide repeats. With the enlarged upper buffer chamber, water circulation system and heat-sinking aluminium ceramic plates, the Twin M provides high speed separation while maintaining straight, sharp bands.

Supplier: VWR Chemicals

Reagent for stabilisation of buffer formulations and electrophoresis of biological molecules.

Supplier: VWR Collection

The shiroGEL vertical 20 offers application flexibility and is distinguished by its ease of gel casting and set-up, requiring only four screws to secure up to four 20×20 cm gels. This vertical screw clamp technology distributes pressure evenly along the height of gel rather than in the centre to eliminate plate bowing and gel compression, while maintaining a leakproof seal during casting. A built-in inner buffer chamber within the PAGE insert allows set up to be completed without inclusion of an upper buffer chamber. These systems are ideal for 1D and 2D SDS-PAGE, native, preparative, gradient and high resolution nucleic acid electrophoresis.

Supplier: VWR Chemicals

Reagent for stabilisation of buffer formulations and electrophoresis of biological molecules.

Supplier: VWR Chemicals

Reagent for stabilisation of buffer formulations and electrophoresis of biological molecules.

Supplier: VWR Collection

The shiroGEL mini vertical PAGE system is modular, allowing PAGE and electroblotting to be carried out in the same tank, simply by changing the inserts. The system features inserts for each application, and a common buffer tank in which to run them. Each insert has its own electrode assembly, which connects to the lid of the buffer tank. For safety, the power leads connect directly to the lid. When the lid is removed, power is disconnected from the system. The inserts also have a small pad that fits into position in the lid. This ensures that the lid is always properly placed and aids in its removal. The buffer tank is moulded to prevent separation and leaking. A variety of spacers, combs and other accessories are available to customise the system. All spacers and combs are colour coded or clearly labelled to indicate thickness.

Supplier: VWR Chemicals

Ethidium bromide solution is a fluorescent stain for visualising nucleic acids in solution or in electrophoresis gels. The solution is provided at a concentration of 0,625 mg/ml. A final working concentration of 0,5 g/ml is easily obtained by adding one drop of solution to 50 ml of molten agarose, running buffer or staining buffer. Ethidium bromide solution in dropper bottles may be stored at room temperature for up to one year.

Supplier: VWR Chemicals

Reagent for stabilisation of buffer formulations and electrophoresis of biological molecules.

Supplier: VWR Collection

Designed for ease of use and safe operation, the shiroGEL horizontal electrophoresis systems and accessories are built to withstand the rigours of everyday use. For leakproof performance the gel boxes and gel trays are moulded from thick acrylic. UV transparent gel trays aid in visualisation of samples. Cassettes protect the electrodes and allow for easy replacement. Gel casting is simple with the durable rubber casting gates. Slots in the sides of the trays allow for easy comb placement. The two-piece design of the combs allows for vertical adjustment, giving the user control over the depth of the well. The combs supplied with the systems are 1,5 mm thick. Multiple combs, including those compatible with multichannel pipettes and different thicknesses, are also available. The 'EasyLift' gel box lid is easily removed using the side tabs and pressure pads. The lid is domed, to prevent condensation from dripping onto the gel, and is surrounded by a drip ring, to help recover the condensate and maintain buffer concentration.

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.