qScript™ cDNA SuperMix, QuantaBio

SuperMix provides a sensitive and easy-to-use solution for two-step qRT-PCR

• Sensitive first-strand cDNA synthesis of RNA sequences ≤1kb
• Single tube, 5X concentrated master mix minimizes pipetting and improve assay accuracy
• Broad linear dynamic detection range with limiting (10 pg) or plentiful (1 µg) samples of total RNA
• Pre-blended with ribonuclease inhibitor protein (RIP) to prevent RNA template degradation during incubation and a proprietary blend of random and oligo(dT) primers, optimized to deliver unbiased representation of 5’ and 3’ sequences

This 5x concentrated master mix provides all necessary components (except RNA template) for first-strand synthesis including: buffer, dNTPs, MgCl2, primers, RNase inhibitor protein, qScript reverse transcriptase, and stabilizers. qScript™i s a RNase H(+) derivative of MMLV-RT, optimized for reliable cDNA synthesis over a wide dynamic range of input RNA. The unique blend of oligo (dT) and random primers in the qScript™ cDNA SuperMix works exceptionally well with a wide variety of targets. This blend is optimized for the production of targets <1kb in length. qScript™ cDNA SuperMix produces excellent results in both real-time and conventional RT-PCR.