1187 Results for: "PfuUltra II Fusion HS DNA Polymerase"
VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix
Supplier: VWR Chemicals
VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.
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Agarose, Electran, high resolution for electrophoresis
Supplier: VWR Chemicals
Agarose high resolution (HR) has melting, max. 90 °C, and gelling temperatures (32,5 to 38 °C), but differs from traditional low melting point molecular biology grade agaroses in the gel separation range. Using this agarose in electrophoresis allows fine and consistent resolution of nucleic acids below 1000 base pairs, which differ by only a few base pairs. The properties of Agarose HR allow consistent gel separations (analytical and preparative) and performance of in-vitro translation and transcription mapping, as well as in-vivo ligation and transformation. For analytical gel separation of DNA/RNA below 1000 base pairs, where a higher gel strength for ease of manipulation is required, we recommend the use of Agarose, high resolution, Electran (43655).
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VWR®, Taq DNA polymerase, 500 U
Supplier: VWR Chemicals
Taq DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of Taq DNA Polymerase to a 25 µl PCR reaction.
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VWR®, Taq DNA polymerase
Supplier: VWR Chemicals
VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.
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VWR®, Taq Plus 2X master mixes
Supplier: VWR Chemicals
VWR® Taq Plus is an optimised format of Taq DNA polymerase master mix and, therefore, is a good alternative to Taq DNA polymerase and Taq DNA polymerase master mix.
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VWR®, Taq DNA polymerase glycerol free
Supplier: VWR Chemicals
VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.
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VWR®, Taq DNA polymerase, glycerol-free
Supplier: VWR Chemicals
Highly concentrated Taq DNA polymerase glycerol free 50 U/µl is ideal for industrial use and manufactures of diagnostic PCR kits.
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VWR®, TEMPase Hot Start DNA polymerase
Supplier: VWR Chemicals
VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR. A glycerol-free TEMPase Hot Start DNA Polymerase is also available for automation and freeze drying.
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VWR®, Red Taq DNA polymerase master mix
Supplier: VWR Chemicals
VWR® Red Taq DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.
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VWR®, Taq DNA polymerase, 200000 U
Supplier: VWR Chemicals
Taq DNA polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from thermus aquaticus and has a molecular weight of approximately 94 kDa.
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VWR®, Red Taq DNA polymerase
Supplier: VWR Chemicals
Red Taq DNA Polymerase is a blend of Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing.
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VWR®, Taq DNA polymerase master mix
Supplier: VWR Chemicals
VWR® Taq DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.
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VWR®, TEMPase Hot Start 2× master mix
Supplier: VWR Chemicals
TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.
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VWR®, PCR buffers
Supplier: VWR Chemicals
An optimal buffer system is critical for the performance of successful PCR. VWR® Taq DNA Polymerase kits generally include two different buffers, Key Buffer and Extra Buffer, which are suited for different PCR needs. All buffers contain Tris and 15 mM MgCl₂ (1,5 mM MgCl₂ final concentration). Additional MgCl₂ for easy optimisation is included in a separate vial.
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Agarose, Electran, high resolution, low melting for electrophoresis
Supplier: VWR Chemicals
For analytical gel separations of DNA/RNA below 1000 base pairs where a higher gel strength for ease of manipulation is required
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Agarose, Electran, wide range, low melting for electrophoresis
Supplier: VWR Chemicals
Agarose, low gelling temperature has low melting and gelling temperatures, low sulphate content and high optical clarity. It is suitable for use in protein and nucleic acid electrophoresis and for immobilisation of heat-labile substances (e.g. bacteria, yeast and eukaryotic cells) due to the low gelling point. In addition, the low melting point of this agarose offers the possibility of recovering proteins and nucleic acids without denaturation when re-melted.
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Betaine enhancer solution 5 M (5X) for PCR
Supplier: VWR Chemicals
Betaine enhancer is especially effective when used with high GC-rich regions or templates with a high degree of secondary structures. It has a decreasing effect on the primer melting temperature.
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VWR®, Melting point standards
Supplier: VWR Chemicals
These standards can be used with any melting point apparatus and are prepared using pure raw materials. Melting points are determined using a high accuracy Differential Scanning Calorimeter (DSC) system that is calibrated to the ITS 90 International Temperature Scale.
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Paraffin, Solidification point: 52-54°C, pellets Ph. Eur.
Supplier: VWR Chemicals
Paraffin, Solidification point: 52-54°C, pellets Ph. Eur.
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Iron(II) sulphate heptahydrate ≥99% ACS
Supplier: VWR Chemicals
Iron(II) sulphate heptahydrate ≥99% ACS
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Palladium(II) chloride (59% Pd) hydrogenation catalyst
Supplier: VWR Chemicals
Palladium(II) chloride (59% Pd) hydrogenation catalyst
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Lead(II) acetate trihydrate 99.5-103.0%, AnalaR NORMAPUR® analytical reagent
Supplier: VWR Chemicals
Lead(II) acetate trihydrate 99.5-103.0%, AnalaR NORMAPUR® analytical reagent
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Mercury(II) sulphate ≥98.0%, BAKER ANALYZED® ACS, J.T.Baker®
Supplier: Avantor
Mercury(II) sulphate ≥98.0%, BAKER ANALYZED® ACS, J.T.Baker®
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Mercury(II) sulphate ≥98.0%, AnalaR NORMAPUR® analytical reagent
Supplier: VWR Chemicals
Mercury(II) sulphate ≥98.0%, AnalaR NORMAPUR® analytical reagent
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Iron(II) chloride tetrahydrate ≥99%, purified
Supplier: VWR Chemicals
Iron(II) chloride tetrahydrate ≥99%, purified
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Copper(II) chloride dihydrate, BAKER ANALYZED® ACS, J.T.Baker®
Supplier: Avantor
Copper(II) chloride dihydrate, BAKER ANALYZED® ACS, J.T.Baker®
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Cobalt(II) nitrate hexahydrate ≥96%, TECHNICAL
Supplier: VWR Chemicals
Cobalt(II) nitrate hexahydrate ≥96%, TECHNICAL
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Cobalt(II) chloride hexahydrate 98.0-102.0%, BAKER ANALYZED® ACS, J.T.Baker®
Supplier: Avantor
Crystals. Lot analysis on label.
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Water, ULTREX® II, Ultrapure, J.T.Baker®
Supplier: Avantor
Water, ULTREX® II, Ultrapure, J.T.Baker®