You searched for: Reagenti per microscopia e colorazione

Supplier: Merck

Microscopy and staining reagents, Immersion oil acc. to ISO 8036 modified

Supplier: Merck

Fixing reagent for enzyme cytochemistry

Supplier: Merck

Neo-Clear® rappresenta l'alternativa a basso rischio allo xilene. Praticamente inodore, compatibile con la maggior parte dei solventi ad elevata purezza e caratterizzato da un tasso di evaporazione inferiore rispetto allo xilene, quindi meno pericoloso per la salute.

Supplier: SIGMA ALDRICH MICROSCOPY

Spurr resin is a hydrophobic resin. It is less viscous than epon mixtures and is easier to dispense. The low viscosity of the resin contributes to its penetration in tissue, mineral and dense structures. A low viscosity of 60 cps also allows easy penetration into a variety of difficult materials.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil has been used in epifluorescent microscopy and cell identification.

Supplier: SIGMA ALDRICH MICROSCOPY

HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites and nucleic acid sites. This formulation in non-citrus, contains no mercury, formaldehyde or glutaraldehyde.

Supplier: Merck

DPX new is a synthetic non aqueous mounting medium for microscopy. In its formulation, the teratogenic plasticiizer dibutyl phthalate (DBP) has been avoided, which renders the composition safer for the environment and more user friendly than the conventionally used mounting agents such as DPX.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

Supplier: SIGMA ALDRICH MICROSCOPY

DPX Mountant for histology has been used as a slide mountant to fix sections.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.

Supplier: SIGMA ALDRICH MICROSCOPY

This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.

Supplier: SIGMA ALDRICH MICROSCOPY

D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

Supplier: Merck

Le soluzioni di montaggio non acquose, ad esempio Neo-Mount®, sono destinate al montaggio permanente e alla conservazione a lungo termine dei campioni. I vetrini processati nei laboratori patologici devono essere conservati fino a 10 anni, di conseguenza i risultati della colorazione devono essere stabili e non possono ammettere scolorimenti. La soluzione di montaggio anidra Neo-Mount® per microscopia è estremamente stabile e prodotta con solventi a base di miscele di idrocarburi alifatici. Poiché contiene un componente privo di aromatici in sostituzione dello xilene, la soluzione Neo-Mount® può essere combinata esclusivamente con Neo-Clear®. Non utilizzare lo xilene in fase di montaggio, in quanto causa l'offuscamento e la striatura dei vetrini. In aggiunta, posizionando i vetrini disidratati sulla carta filtrante per circa 1 minuto prima del montaggio, si impedisce l'eventuale eccesso di Neo-Clear®, in quanto sotto il vetrino coprioggetto potrebbero formarsi bolle di aria. Tale azione preventiva è da eseguire anche quando si montano i vetrini con macchine montavetrini; in quest'area, un'efficace eliminazione di NEO-CLEAR® si ottiene incubando i vetrini per un minuto in un rack per vetrini vuoto.

Supplier: Merck

Entellan® new è una nuova soluzione di montaggio per montavetrini ed è particolarmente adatta per strumenti commerciali di montaggio automatizzati utilizzati unitamente a montavetrini. Ha un range di viscosità di 250 - 600 mPas, il cod. prod. 1,00869 ha un range inferiore. Questo basso range di viscosità migliora e accelera l'applicazione, in quanto non occorre ripristinare il tasso di erogazione o il volume dopo il primo ciclo con un nuovo flacone di Entellan® new.

Supplier: Merck

Aquatex is used for e.g. LEUCOGNOST stained specimens. For preservation purposes and subsequent optimal microscopy, the stained sections are normally mounted using a suitable mounting agent and covered with a cover glass.

Supplier: Merck

Entellan® New is a new rapid mounting medium for cover slippers and is specially suited for commercial automated mounting instruments that operate with cover slippers.

Supplier: SIGMA ALDRICH MICROSCOPY

For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: Merck

M-FIX is a fixative spray designed for the wet fixation of gynaecological and non-gynaecological preparations processed in Papanicolaou staining. It contains polyglycols in alcoholic solution and a rapid and secure fixation of specimens is possible.

Supplier: SIGMA ALDRICH MICROSCOPY

JB-4 is a water-soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. It is a technique now widely used for research and clinical diagnosis. JB-4 yields semi-thin sections (0.5μ-2μ) with excellent morphological preservation.

Supplier: SIGMA ALDRICH MICROSCOPY

Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.

Supplier: SIGMA ALDRICH MICROSCOPY

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination

Supplier: SIGMA ALDRICH MICROSCOPY

Epoxy embedding medium is generally used for biological tissues during study by electron microscopy. Usually, it consists of three components; Epon 812 resin, an anhydride hardener and an accelerator

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with PPD (p-phenylenediamine; 1,4-phenylenediamine) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

Supplier: SIGMA ALDRICH MICROSCOPY

To be used with SIAL 69571 Ren HY 956 (Hardener for Epoxy Embedding Medium)