buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
EDTA, 0.5M pH 8.0, Corning®
Supplier: Corning
EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.
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ISE sample conditioning buffers and solutions
Supplier: WTW
Ion strength adjustment solutions have multiple tasks: TISAB for fluoride measurements does not only adjust the pH value, by also decomplexes the fluoride-containing complex compounds. Other ion strength adjustment solutions ensure an adapted pH value as well as the addition of a suitable conducting salt to correctly capture the concentration.
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Buffer solutions
Supplier: REAGECON
Buffer solution, pH value: 4,00, ±0,01, Reference temperature: 25 °C, Plastic bottle
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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants
Supplier: G-Biosciences
ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.
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Tris Hydrochloride
Supplier: Merck Millipore (Calbiochem)
Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).
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Protein extraction reagent, BugBuster® HT, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.
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pH buffer solutions, colour coded
Supplier: REAGECON
Colour coded pH buffer solutions for the calibration of pH meters.
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Hank's balanced salt solution, modified, MP Biomedicals
Supplier: MP Biomedicals
Cell culture buffer, Hank's balanced salt solution, modified (1X)
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Carbonate Borax Free Buffer Solution
Supplier: REAGECON
This range of Reagecon pH Buffers are sodium tetraborate (Borax) free. They are not subject to classification by EU regulatory authorities as toxic to reproduction under CLP category 1B. Similar in approach to the existing range of Reagecon Mercury Free Buffers, these Buffer Solutions have been developed to enhance user choice and allay safety concerns, without any impact on fitness for purpose or product performance.
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pH electrode calibration and maintenance solutions kit
Supplier: WTW
Adapted to different electrode types for calibration and maintenance.
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Hank's balanced salts, MP Biomedicals
Supplier: MP Biomedicals
Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals
Supplier: MP Biomedicals
Used to minimise cell attachment or aggregation.
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Saline solutions
Supplier: BALIS
Cell culture buffer, Physiological saline solution, 9 ml, no standards
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Buffer tablets, Lovibond®
Supplier: Lovibond
Ideal for preparing buffer solutions of 4, 7 and 10 pH units.
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pH buffer solutions
Supplier: Mettler - Toledo
Technical buffer solution, 250 ml, pH value: 7,00
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Redox buffer solutions
Supplier: Mettler - Toledo
Redox buffer solutions are used for verification of all common redox sensors.
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