buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Mammalian protein extract reagents, M-PER™

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

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Buffers, TBS, 20X solution, pH 7,4, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Used in Western blotting protocols.

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Subcellular Protein Fractionation Kit for Cultured Cells

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

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Cesium chloride - lanthanum chloride buffer, for flame AAS, TraceSELECT™, Fluka™

Supplier: Honeywell Chemicals

TraceSELECT™, for flame atomic absorption spectrometry.

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HEPES Buffer, 1 M Sterile, Cell Culture Grade, MP Biomedicals

Supplier: MP Biomedicals

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.

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Y-PER™ Yeast Protein Extraction Reagent

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

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CSPL buffer

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

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Ab buffer kit

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

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$Subcellular protein fractionation kit for tissues$

Subcellular protein fractionation kit for tissues

Supplier: Thermo Fisher Scientific

This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

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Rapid-hyb buffer

Supplier: Cytiva

A major limitation of membrane hybridisation is speed. At commonly used probe concentrations (2 to 10 ng/ml), hybridisation normally takes 16 hours to detect single-copy gene sequences in mammalian DNA. Use of Rapid-hyb buffer reduces this time to 2 hours using similar probe concentrations.

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PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

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Protein A/G HP SpinTrap™ Buffer Kit

Supplier: Cytiva

Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.

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Mini flip-top vial with 9 ml Letheen Broth

Supplier: 3M Food Safety

The 3M™ Mini Flip-Top Vial with 9 ml Letheen Broth is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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Buffer, PBS solution 10X concentrate (phosphate buffered saline), MP Biomedicals

Supplier: MP Biomedicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Low pH SDS Sample Buffer

Supplier: EKSIGENT

SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.