buffers

Supplier: G-Biosciences

BLOK™ BSA is a 10% BSA protein blocking solution.

Supplier: Thermo Fisher Scientific

Sodium acetate buffer solution pH 5,2 is been widely used in for precipitation of nucleic acids and preparation of gel stains for protein gel electrophoresis.

Supplier: G-Biosciences

A 10X concentrated Tris Buffered Saline supplemented with Tween 20.

Supplier: Cytiva

DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.

Supplier: Thermo Fisher Scientific

Cloning Buffer, Potassium acetate, 1M aq, solution, pH 7,5, RNAse free

Supplier: Cytiva

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.

Supplier: G-Biosciences

BLOK™ BLOTTO is a 5% novel modified milk protein solution used for Westerns, ELISA and dot blot blocking.

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

Supplier: Honeywell Chemicals

TISAB according to DEV D4.

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

Supplier: Corning

20X Saline-Sodium-Phosphate-EDTA (SSPE) buffer has applications in nucleic acid hybridization and other molecular biology work.

Supplier: G-Biosciences

Water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH.

Supplier: Honeywell Chemicals

Matrix Modifier, 2% Potassium (K) in 1% HCl 1%

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

Supplier: HyClone products (Cytiva)

Balanced salt solution for cell culture applications.

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.