buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
G-Biosciences Acetate Buffer
Supplier: G-Biosciences
Acetate buffer at pH 5,0 and 5,5 are supplied as ready to use solutions that eliminate the calculation, formulation and weighing errors.
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TISAB II Solution, Supelco®
Supplier: Merck
TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.
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Proteometer-L fluorescent reagent
Supplier: NOVILYTIC
7 vials of the Proteometer-L fluorescent reagent specifically formulated for use with Proteometer-L kit.
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Proteometer-CV reconstitution reagent
Supplier: NOVILYTIC
1 x vial of reconstitution reagent.
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Hank's balanced salt solutions (HBSS), Corning®
Supplier: Corning
Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO₂ atmospheric conditions.
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Krebs-Ringer solution, HEPES-buffered
Supplier: Thermo Fisher Scientific
Contains 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, 5,5 mM HEPES, and 1 mM D-glucose
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Dulbecco's phosphate buffered saline (DPBS), ES cell qualified, HyClone™ AdvanceSTEM™
Supplier: HyClone products (Cytiva)
A balanced salt solution tested for use with embryonic stem cells.
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Protein extraction kit, tissue PE LB™
Supplier: G-Biosciences
Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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Buffer, PBS tablets, pH 7,4, Millipore®
Supplier: Merck Millipore (Oncogene)
Developed for use in immunoassay applications.
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Buffer, SSC 20X powder for molecular biology, MP Biomedicals
Supplier: MP Biomedicals
SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.
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Krebs-Ringer solution, bicarbonate-buffered
Supplier: Thermo Fisher Scientific
Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.
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Protein extraction kit, mammalian cell PE LB™
Supplier: G-Biosciences
Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
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Proteometer-UFT fluorescent reagent
Supplier: NOVILYTIC
3 vials of Proteometer-UFT Fluorescent reagent specifically formulated for use with Proteometer-UFT kit.
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Sodium Cacodylate, 0,1 M Buffer solution, pH 6,5
Supplier: Thermo Fisher Scientific
Liquid.
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Proteometer-CV mobile phase A salts
Supplier: NOVILYTIC
5 sealed salt packets specifically formulated for use with Proteometer-CV kit.
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