buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

CHAPS lysis buffer

Supplier: Thermo Fisher Scientific

For cell lysis and protein extraction.

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LDS-sample buffer (4X), non-reducing

Supplier: Thermo Fisher Scientific

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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Acetate buffer solution, Fluka™

Supplier: Honeywell Chemicals

This pH buffer solution is a combination of sodium acetate/acetic acid in 1:1 ratio for complexometry.

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Buffers, concentrated solutions, Fluka™

Supplier: Honeywell Chemicals

pH Buffer, concentrate, pH value: 10,00, ±0.05, Ampoule

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Tris-Acetate-EDTA (TAE) buffer, 50X

Supplier: PanReac AppliChem

50X concentrated solution of 2 M Tris, 1 M glacial acetic acid and 0,5 M EDTA.Na₂, pH 8,5 ±0,2.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Supplier: Corning

20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures

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Proteometer-UFT kit

Supplier: NOVILYTIC

The Proteometer-UFT Kit transforms any LC into an analytical system capable of identifying and quantifying titer content in crude culture filtrate in <90 seconds, without sample preparation.

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Buffer, BIS-TRIS propane, 0,2 M solution, pH 6,5

Supplier: Thermo Fisher Scientific

BIS-TRIS propane buffer solution pH 6,5 (0,5 mol/l), Liquid

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Proteometer-UFT starter kit

Supplier: NOVILYTIC

The Proteometer-UFT Kit transforms any LC into an analytical system capable of identifying and quantifying titer content in crude culture filtrate in <90 seconds, without sample preparation.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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1M tris-hydrochloride buffers, Corning®

Supplier: Corning

Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification.

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TE buffer, pH 8,1, RNAse free

Supplier: Thermo Fisher Scientific

Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.