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"Staining Solutions"

 
 

Methylene blue trihydrate, powder C.I. 52015

Supplier: MP Biomedicals

Methylene blue has been used as a stain in bacteriology and as an oxidation-reduction indicator. A sensitive stain for RNA and
ribonuclease. An ethidium bromide alternative for the visualization of nucleic acids in gels:

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Sodium hydroxide 4 mol/l (4 N) in aqueous solution, VOLUSOL® volumetric solution

Sodium hydroxide 4 mol/l (4 N) in aqueous solution, VOLUSOL® volumetric solution

Supplier: VWR Chemicals

Sodium hydroxide 4 mol/l (4 N) in aqueous solution, VOLUSOL® volumetric solution

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Conductivity standards, Potassium chloride, IUPAC

Conductivity standards, Potassium chloride, IUPAC

Supplier: Hach

Potassium chloride standard solutions according to OIML, traceable to NIST and Standard solution verified by Reference Materials Laboratory.

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Maltose

Supplier: Apollo Scientific

Maltose

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Ponceau S, Bioreagent for electrophoresis, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The following are two common stain formulations: 0.1% Ponceau S (w/v) in 5% acetic acid 2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid.

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2,2’-sulphonyldiethanol solution 65% in water

Supplier: Apollo Scientific

2,2’-sulphonyldiethanol

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Plasmid DNA buffers

Supplier: OMEGA BIO-TEK

Solution 1, resuspension buffer

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Fuchsin (acid), pure, high purity biological stain

Fuchsin (acid), pure, high purity biological stain

Supplier: Thermo Fisher Scientific

Fuchsin (acid), pure, high purity biological stain

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Orange G 85+%, pure, high purity biological stain

Orange G 85+%, pure, high purity biological stain

Supplier: Thermo Fisher Scientific

Orange G 85+%, pure, high purity biological stain

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NHS Ester, SureLight® 488 molecular biology stain

NHS Ester, SureLight® 488 molecular biology stain

Supplier: Columbia Biosciences

SureLight® 488 NHS Ester is a bright green fluorescent dye with excitation suited to the 488nm laser line, fluorescent microscopy and flow cytometry. It offers a much more stable signal generation in imaging and flow cytometry than FITC and is half the cost of Alexa Fluor® 488 with no royalties or licensing fees.

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Potassium bromate 0,100 N in aqueous solution 1 N in aqueous solution USP Test Solution (TS) 34
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Hydrochloric acid 2 N, BAKER ANALYZED® volumetric solution, J.T.Baker®

Hydrochloric acid 2 N, BAKER ANALYZED® volumetric solution, J.T.Baker®

Supplier: Avantor

Standardised at 25 °C against chemicals whose certification is traceable to NIST. Lot standardisation value, accurate to ±1 part per 1000, provided on label.

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Indigo carmine, high purity biological stain, for analysis

Indigo carmine, high purity biological stain, for analysis

Supplier: Thermo Fisher Scientific

Indigo carmine, high purity biological stain, for analysis

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Cell culture salts, salt solutions and buffers

Cell culture salts, salt solutions and buffers

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

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Conductivity standard 1413 µS/cm for conductivity measurement

Supplier: KNICK

KCl solution, 1,413 µS/cm , Reference temperature: 25 °C

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Conductivity calibration solutions, YSI

Conductivity calibration solutions, YSI

Supplier: YSI ENVIRONMENTAL

Conductivity standard, 50 000 µS/cm

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