"Staining Solutions"
G418 solution
Supplier: Biowest
Antibiotic G418 is an aminoglycoside antibiotic similar in structure to gentamycin. It exhibits toxicity towards both eukaryotic and prokaryotic cells. The optimal concentration for selection and maintenance must be determined for each cell line.
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Eosin Y (yellowish), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Eosin Y (yellowish), Sigma-Aldrich®
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Dirt and stain remover, Miraclean
Supplier: FHP VILEDA SCS
This dirt and stain remover made from 100% melamine resin can be used for removing tough marks, stains and ground-in dirt.
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CytoFix™ Red Lysosomal Stain
Supplier: AAT Bioquest
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste and cellular debris through a process known as autophagy.
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Proteinase K solution
Supplier: PanReac AppliChem
Proteinase K is used to destruct proteins in cell lysates (tissue, cultured cells) and to liberate nucleic acids, since it very effectively digests DNases and RNases.
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Collodion 2% in amyl acetate for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Solution 2% in amyl acetate (Cellulose nitrate).
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Bench liner rolls and sheets, Labmat™, SP Bel-Art
Supplier: Bel-Art Products, a Part of SP
Disposable Labmat™ bench liners speed clean-up and safeguard work surfaces from stains, spills and wear. Simply roll up and discard after contamination.
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Sodium bicarbonate : Sodium carbonate concentrate (64 mM : 20 mM) in water IC eluent concentrate (20x) for Metrosep A Supp 5, Supelco®
Supplier: Merck
The “bicarbonate concentrate” is 1-molar solution of sodium bicarbonate (the saturation temperature of the 1-molar solution is 15°C). It is highly advantageous as it is temperature dependent and its subsequent dilution with water can be appropriately controlled
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Sodium borohydride 12% in aqueous solution, VenPure™
Supplier: Thermo Fisher Scientific
Sodium borohydride 12% in aqueous solution, VenPure™, pure
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Formaldehyde 16% (w/v) methanol-free, Pierce™
Supplier: Thermo Fisher Scientific
Methanol-free formaldehyde is a highly reactive, cell-permeable agent that is used by researchers as a reversible crosslinking agent for proteins and nucleic acids within the cell or as a general cell-fixing agent for imaging-based applications.
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D-(-)-Sorbitol solution 1 M
Supplier: G-Biosciences
Quality DNase and RNase free reagent solution for yeast research.
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Propidium iodide 1 mg/ml in aqueous solution
Supplier: AAT Bioquest
Propidium iodide (PI) is a red-fluorescent dye widely used for staining and identifying dead cell populations in flow cytometry and fluorescence microscopy.
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Potassium dihydrogen phosphate 1/15 mol/l in aqueous solution, Supelco®
Supplier: Merck
Potassium dihydrogen phosphate 1/15 mol/l in aqueous solution, Supelco®
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Accessories for Memmert HCP humidity chambers
Supplier: MEMMERT
Stainless steel perforated shelf, For: HCP50
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Conductivity standards, Potassium chloride, IUPAC
Supplier: Hach
Potassium chloride standard solutions according to OIML, traceable to NIST and Standard solution verified by Reference Materials Laboratory.
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Leishman's eosin-methylene blue, greenish-black powder
Supplier: MP Biomedicals
Leishman's stain, also Leishman stain, is used in microscopy for staining blood smears. It provides excellent stain quality. It is based on a methanolic mixture of "polychromed" methylene blue (i.e. demethylated into various azures) and eosin.
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