"Staining Solutions"

Supplier: Merck

Hydrochloric acid in ethanol is used for the microscopical investigation of mycobacteria in sample material of human origin. It can be applied as decolorizing solution, as e.g. in the Tb-color staining set.

Supplier: SIGMA ALDRICH MICROSCOPY

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

Supplier: Abcam

Cationic dye. Forms insoluble complexes with glycosaminoglycans in solution. Stains interphase nuclei, especially mitotic figures.

Supplier: SIGMA ALDRICH MICROSCOPY

Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.
Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.

Supplier: G-Biosciences

The stain uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied agent first oxidises the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive glycoprotein stain solution forming Schiff bonds and producing strong magenta colour bands. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining. The glycoprotein stainis highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included.

Supplier: VWR Chemicals

Staining protein after electrophoretic separation. Also used to determine chloride ions in solution.

Supplier: SIGMA ALDRICH MICROSCOPY

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Supplier: G-Biosciences

G-Biosciences' Coomassie Brilliant Blue (CBB) G-250 and R-250 Stain are based on a colloidal Coomassie stain. CBB G-250 and R-250 stain proteins with high band visibility. The staining of gels with CBB G-250 and R-250 allows the examination of protein bands even during the staining process.

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

Supplier: G-Biosciences

Trypan Blue 0.4% Solution is commonly used as a cell stain to measure cell viability using dye exclusion method. The dye exclusion method is based on the principle that cell impermeable dyes like Trypan blue will stain only dead cells where as viable cells will not be stained.

Supplier: G-Biosciences

G-Biosciences' Coomassie Brilliant Blue (CBB) G-250 and R-250 Stain are based on a colloidal Coomassie stain. CBB G-250 and R-250 stain proteins with high band visibility. The staining of gels with CBB G-250 and R-250 allows the examination of protein bands even during the staining process.

Supplier: Merck

Demonstration of elastic fibers

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde and 0,01% citric acid in aqueous solution.

Supplier: Abcam

Abcam's Flow Cytometry Lysing Solution is a ready-to-use solution formulated for lysing erythrocytes following monoclonal antibody staining of whole blood.

Supplier: Abcam

Nuclear fast red stain solution (pH 2 - 4) is a stain for histological applications. - Stains nuclei red and cytoplasm pale pink - Contains 80% water, 5% aluminium sulfate, 0.2% nuclear fast red - Enhanced stability - Prevents precipitation over time