"Staining Solutions"
Pierce™ Silver Stain Rescue Reagent
Supplier: Thermo Fisher Scientific
Pierce silver stain rescue reagent enables removal of undesirable background from unevenly and overly developed silver-stained polyacrylamide electrophoresis gels.
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Mounting Medium, CC/Mount™
Supplier: SIGMA ALDRICH MICROSCOPY
CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.
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System for parasitological examination of stool, ParasiTrap® AF
Supplier: Biosepar
ParasiTrap® enables detection of worm eggs, protozoa and larvae. The ParasiTrap® is a transport and testing system for the parasitological examination of stools in human and veterinary medicine. It is a completely closed system with 4 simple steps to make the diagnosis: 1. load tube with sample; 2. add solvent and stain; 3. filter and concentrate; 4. examine under microscope. It was developed to facilitate the unpleasant task of processing infectious stools in laboratory environments, reduce risk of infection and ensure the best possible yield of parasites. The closed system also allows macroscopic diagnostic procedures to be performed where necessary. This diagnostics concept fully complies with the modern quality management criteria of national and international guidelines (EU, CDC, WHO, BGA, DPG). All systems are free of merthiolate and ether. The ParasiTrap® AF-System contains a filled processing and transport tube I (with Medium AF) and the processing tube II with integrated stainless steel filter system. Accessories: separation medium B, staining solution medium C, Pasteur pipettes (disposable), spoons and cotton wool sticks. Additional screw caps for processing tube II.
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G-Biosciences Sputo-LR™ Sputum Liquefying Reagent
Supplier: G-Biosciences
Sputo-LR™ is a concentrated sterile solution of Cleland’s reagent (dithiothreitol, DTT) in phosphate buffer at pH 7,0 and is for processing samples from sputum for smears and culture.
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4',6'-Diamidino-2-phenylindole dilactate (DAPI dilactate) fluorescent dye, used in nuclear staining
Supplier: Biotium
DAPI dilactate is essentially the same as DAPI dihydrochloride (catalog no. 40011), but with a different counterion. The dilactate salt form of the dye is more water soluble than the dihydrochloride salt, and therefore DAPI dilactate is a better choice for preparing stock solutions in water.
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VWR® Q Path® Coverquick, Mounting Agents for Microscopy
Supplier: VWR Chemicals
A range of mounting media for manual and automatic mounting of slides. The Q Path® Coverquick range consists of four different mounting media with different viscosities to meet all microscopy needs. The number after the name indicates increasing viscosity: Q Path® Coverquick 1000 has the lower viscosity (around 260 mPaS) and Q Path® Coverquick 4000 has the higher viscosity (around 920 mPaS).
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StayYellow/HRP, Abcam
Supplier: Abcam
StayYellow/HRP is a substrate chromogen system designed to be used for either IHC or ISH when using HRP detection.
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StayGreen/AP Plus (Alcohol and Xylene Substitute Compatible), Abcam
Supplier: Abcam
StayGreen/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection.
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Bouin's fluids
Supplier: CLIN TECH
This staining fixative is stable under normal temperature conditions and recommended use.
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Eosin B 1 g/l in aqueous solution, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Eosin B 1 g/l in aqueous solution, Sigma-Aldrich®
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Gram's safranine solution for the Gram staining method 1 * 25 L
Supplier: Merck
Gram's safranine solution for the Gram staining method 1 * 25 L
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Glycerol Mounting Medium - Anti-Fade with DAPI, Abcam
Supplier: Abcam
Glycerol Mounting Medium with DAPI is an aqueous anti-fade mounting medium containing DABCO for preserving fluorescence of tissue and cell smears.
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Mounting Medium With PI - Aqueous, Fluoroshield, Abcam
Supplier: Abcam
Mounting Medium with PI (Propidium iodide) ab104129 (previously called Fluoroshield Mounting Medium with Propidium Iodide) is an aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples.
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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™
Supplier: Biotium
EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.
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Methylene blue (Neisser) for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Methylene blue, Neisser formulation. Well developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractive bodies within the bacterial cytoplasm. With methylene blue they stain metachromatically, a reddish purple color.
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Epoxy Embedding Medium Kit
Supplier: SIGMA ALDRICH MICROSCOPY
The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).
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