"Staining Solutions"
Xylene substitute, Neo-Clear®, Sigma-Aldrich®
Supplier: Merck
Neo-Clear® is a non hazardous alternative to xylene. It is virtually odourless, compatible with most high purity solvents and is characterised by lower evaporation rates than xylene, making it a lower health risk.
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Embedding and mounting media
Supplier: Merck
The defined hardness and uniformity of the material to be cut are vital requirements in creating consistent quality in cutting. These requirements can be met using a range of suitable substances, such as paraffins, resins, gelatine and celloidin.
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VWR® Q Path® Isomount 2000, Mounting Media
Supplier: VWR Chemicals
Mounting media based on isopropyl alcohol. Compatible with most solvent replacing agents. Suitable for manual and automatic mounting of slides. PET bottle of 500 ml.
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Eukitt® mounting medium
Supplier: SIGMA ALDRICH MICROSCOPY
This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.
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Araldite® M, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.
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Mounting Medium for IHC - Aqueous, Abcam
Supplier: Abcam
Aqueous mounting medium for IHC is designed for use with chromogenic tissue and cell staining. - Works with both aqueous and non-aqueous chromogens (e.g., Fast-Red, AEC, DAB). - Forms a dry, even surface for direct visualization without glass coverslip
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Immersion Oil BioChemica
Supplier: Apollo Scientific
Immersion Oil BioChemica
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Eosin Y in aqueous solution
Supplier: Abcam
Eosin Y Solution (Aqueous) ab246825 is intended for use in the histological demonstration of cytoplasm and is commonly used as a counterstain for Hematoxylin.
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VWR®, OCT Embedding matrix for frozen sections
Supplier: VWR Chemicals
When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.
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Oil red O 0.5% in isopropanol (IPA), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.
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Embedding kit JB-4, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
JB-4 is a water-soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. It is a technique now widely used for research and clinical diagnosis. JB-4 yields semi-thin sections (0.5μ-2μ) with excellent morphological preservation.
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Tissue-clearing reagent, TOMEI for plants
Supplier: TCI
Specifications
Appearance: Colorless to Light yellow clear liquid
Functionality test (transparency) (display): to pass test
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Spurr low viscosity embedding kit, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Spurr resin is a hydrophobic resin. It is less viscous than epon mixtures and is easier to dispense. The low viscosity of the resin contributes to its penetration in tissue, mineral and dense structures. A low viscosity of 60 cps also allows easy penetration into a variety of difficult materials.
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DPX, Mounting Medium Fast (Toluene Base) for Clinical Diagnosis
Supplier: Apollo Scientific
DPX, Mounting Medium Fast (Toluene Base) for Clinical Diagnosis
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Non-aqueous DPX mounting medium
Supplier: Merck
DPX new is a synthetic non aqueous mounting medium for microscopy. In its formulation, the teratogenic plasticiizer dibutyl phthalate (DBP) has been avoided, which renders the composition safer for the environment and more user friendly than the conventionally used mounting agents such as DPX.
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Tissue-clearing reagent, CUBIC-R+ for animals
Supplier: TCI
Specifications
Appearance: Colorless to Light orange to Yellow clear liquid
Functionality test: to pass test (Remaining fluorescence strength)
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