"Staining Solutions"

Supplier: MP Biomedicals

Alcian Blue 8GX is a cationic dye used to quantitatively determine glycosaminoglycans by forming insoluble complexes in solution.

Supplier: SIGMA ALDRICH MICROSCOPY

HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites and nucleic acid sites. This formulation in non-citrus, contains no mercury, formaldehyde or glutaraldehyde.

Supplier: SIGMA ALDRICH MICROSCOPY

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

Supplier: VWR Chemicals

HistoChoice® clearing agent is a much less hazardous replacement for xylene/toluene.

Supplier: SIGMA ALDRICH MICROSCOPY

Epoxy embedding medium is generally used for biological tissues during study by electron microscopy. Usually, it consists of three components; Epon 812 resin, an anhydride hardener and an accelerator

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable for use with large and small mineralized (undecalcified) bone sections. Yields clear, hard blocks for cutting sections. Not water-soluble.

Supplier: VWR Chemicals

Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.

Supplier: SIGMA ALDRICH MICROSCOPY

ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.

Supplier: VWR Chemicals

Q Path Labofix is a spray cytology fixative able to fix and store the cellular elements from gynaecological and non gynaecological smears after spreading them out on a glass slide.

Supplier: Abcam

Flow Cytometry Fixation & Permeabilization Kit ab314689 contains optimally formulated buffers for fixation and permeabilization of cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry.

Supplier: SIGMA ALDRICH MICROSCOPY

Glycerol alters the hygroscopic property of gelatin and its permeability to water vapour. Glycerol gelatin or glycerine jelly medium is an aqueous slide mounting media, used for mounting samples in microscopy and histology.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion Oil has a defined refractive index of about 1,516. It is important that the refractive index (nD) is about 1,5, similar to that of glass. This enables a homogeneous oil immersion to be achieved.

Supplier: VWR Chemicals

Fixative, combining the coagulant qualities of alcohol, to enable a better fixation of nuclear and cytoplasmic elements. The erythrocytes are lysed and, therefore, do not obscure observation of the elements of interest. This fixative can be used according to the Saccomanno method.

Supplier: Merck

Entellan® New is a new rapid mounting medium for cover slippers and is specially suited for commercial automated mounting instruments that operate with cover slippers.