"Staining Solutions"

Supplier: VWR Chemicals

Ready to use periodic acid for histological staining in easy to use and safe pouch.

Supplier: VWR Collection

Immersion oil is used to increase microscope resolution.

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil has been used in epifluorescent microscopy and cell identification.

Supplier: VWR Chemicals

High viscosity mounting media for cryotomy. Less viscous than O.C.T.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with PPD (p-phenylenediamine; 1,4-phenylenediamine) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: Merck

Kaiser's glycerol gelatin is an aqueous mounting medium for microscopy and works well with specimens prepared for enzyme, lipid, and immunohistochemistry determinations.

Supplier: Abcam

Fast Red Substrate kit ab64254 for immunohistochemical staining using alkaline phosphatase-based detection.

Supplier: MP Biomedicals

Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. A 10mg/mL easy-to-use solution of ethidium bromide in specially filtered, deionized water which is excellent for nucleic acid electrophoresis and purification applications, eliminates the dust hazard associated with powdered ethidium bromide and saves time spent on weighing and mixing. Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands. Biochem/physiol Actions: Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

Supplier: SIGMA ALDRICH MICROSCOPY

to be used with SIAL 78711 RenLam M-1 (Epoxy Embedding Medium)

Supplier: SIGMA ALDRICH MICROSCOPY

To be used with SIAL 69571 Ren HY 956 (Hardener for Epoxy Embedding Medium)

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.

Supplier: MP Biomedicals

Alcian Blue 8GX is a cationic dye used to quantitatively determine glycosaminoglycans by forming insoluble complexes in solution.