"Staining Solutions"
Aqueous mounting medium, Fluoromount™, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.
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Ethidium bromide Greenbag™ disposal kit, MP Biomedicals
Supplier: MP Biomedicals
The EtBr Greenbag™ Disposal Kit allows rapid and trouble-free concentration of ethdium bromide from large volumes of solutions into a small 'teabag' containing activated carbon. The Greenbag™ is then conveniently disposed of along with other solid hazardous wastes.
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Methylene blue solution, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm.
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Automated cell counter, ADAM-MC2
Supplier: NANOENTEK
ADAM-MC2 is an automated fluorescence cell counter that performs cell counting and viability measurements at highest accuracy capable of replacing complex flow cytometry workflows. Combined with the disposable micro-fluidic Accuchip, operation is extremely simple and cost-effective. Utilising the PI (Propidium Iodide) nucleus staining method, most advanced LED optics and CMOS detection technology, ADAM-MC2 is the perfect solution when working with aggregated, irregular shaped or challenging cells like CAR-T cells, CAR-NK cells or adipose-derived stem cells.
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3D Clear tissue clearing reagents and buffers, Corning®
Supplier: Corning
In our Starter Kit we have combined 30 ml of Corning 3D Clear with the four buffer solutions required to process 3D tissue cultures for immunolabelling. All you need to do is add your immunolabels and nuclear stains. This kit comes with enough material to process three 96-well plates of 3D cell culture models.
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Anti-BrdU Mouse Monoclonal Antibody (FITC) [clone: PRB-1]
Supplier: Tonbo Biosciences
The incorporation of BrdU into newly synthesized DNA by actively cycling cells is one method for measuring the changing amount of cellular DNA during cell proliferation through each of the cell cycle phases. As a thymidine analog, BrdU is preferentially incorporated into newly replicated DNA which can then be subsequently labeled and analyzed to determine relative DNA content and cell cycle position. Incorporation of BrdU is most commonly detected using anti-BrdU antibodies. A BrdU solution is provided for exposure of actively cycling cells to incorporate BrdU. The EZ-BrdU Kit employs an acid denaturation step. The mild acid method used helps reduce damage to other cellular proteins. After the denaturation step, cells are stained with a FITC anti-BrdU antibody and total DNA is counterstained with a PI/RNase A solution. Two color flow cytometry can then be used to analyze cells that have incorporated BrdU (proliferating cells) in terms of their cell cycle position (G0/1, S, or G2/M phase). The EZ-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.
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Neutral mounting medium, DPX, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.
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Eosin Y (yellowish) 1% alcoholic solution counterstain in H&E, for histology
Supplier: Merck
The hematoxylin and eosin (H&E) staining method is the method most frequently used for the staining of histology material of human origin.
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Malachite green oxalate solution, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Staining of bacterial spores according to Schaeffer and Fulton; Pappenheim stain when combined with Gram′s stain
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Periodic acid solution, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
For use in procedure no. 395 for Periodic Acid-Schiff (PAS) and Silver Stain Procedure No. HT100.
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VWR® Gurr™ Histological Fixatives, AFA
Supplier: VWR Chemicals
Acidified alcoholic formalin for the fixation of glycogen.
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LEUCOGNOST® Fixing mixture, Sigma-Aldrich®
Supplier: Merck
Fixing reagent for enzyme cytochemistry
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VWR® Q Path® Easyfix, Cytology Fixative
Supplier: VWR Chemicals
Cytology fixative specially designed for the conservation and transport of cytology specimens.
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VWR® Q Path®, Medium-acting decalcifiers
Supplier: VWR Chemicals
This medium-acting decalcifier contains a mixture of hydrochloric acids.
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Immersion oil acc. to ISO 8036 modified for microscopy (contains dibutyl phthalate and chloroparaffin)
Supplier: Merck
Microscopy and staining reagents, Immersion oil acc. to ISO 8036 modified
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VWR® Q Path® DC3, Decalcifier Rapid-Acting
Supplier: VWR Chemicals
Contains a mixture of hydrochloric acid and alcohols with some EDTA.
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