"Staining Solutions"

Supplier: VWR Chemicals

Destaining bags are designed to safely remove ethidium bromide from solution during overnight treatment. Each bag extracts up to 5 mg of ethidium bromide from solution, thus minimising the exposure of research personnel to this toxic material. The special absorbent mixture retains dye molecules in a convenient bag for removal and incineration.

Supplier: VWR Chemicals

A pre-mixed dye solution used in cell culture applications to determine cell viability. A researcher can remove a sample of cells from culture and combine in a 1:1 ratio with the Trypan Blue solution. Under the microscope, dead cells will appear a blue color and viable cells will appear clear and translucent. Using a hemacytometer (gridded microscope slide), a researcher can quantify the percentage of dead cells within a population. Stained cells are ready for counting within five minutes.

Supplier: HyClone products (Cytiva)

Trypan Blue solution, 0,4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test.

Supplier: AAT Bioquest

DAPI is a fluorescent stain that binds strongly to DNA.

Supplier: Thermo Fisher Scientific

6X DNA Loading Buffer is a pre-mixed solution used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels.

Supplier: PanReac AppliChem

Azur-eosin-methylene blue solution according to Giemsa (slow) for clinical diagnosis.

Supplier: CLIN TECH

Papanicolaou's Orange G solution (OG 6)

Supplier: Tonbo Biosciences

The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.

Supplier: Thermo Fisher Scientific

Wright's stain for microscopical staining

Supplier: Thermo Fisher Scientific

Ethidium bromide destaining bags are used to safely remove ethidium bromide from nucleotide solutions and gels during overnight treatment. Each bag extracts up to 5 mg of Ethidium Bromide from solution, thus minimising the exposure of research personnel to this material.

Supplier: Merck

Aqueous solution containing tris(hydroxymethyl)aminomethane, tris(hydroxymethyl)aminomethane hydrochloride, polyvinyl alcohol 22000, glycerol, and 1,4-diazabicyclo[2.2.2]octane.

Supplier: SIGMA ALDRICH MICROSCOPY

For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

Supplier: Merck

Aquatex is used for e.g. LEUCOGNOST stained specimens. For preservation purposes and subsequent optimal microscopy, the stained sections are normally mounted using a suitable mounting agent and covered with a cover glass.

Supplier: Merck

Counterstain in H&E

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.

Supplier: G-Biosciences

The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.