"SIGMA ALDRICH MICROSCOPY"

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Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.

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2,3-Diaminonaphthalene is also known as 2,3-Naphthalenediamine or DAN. It is one of the isomers of naphthalene bearing two amino groups, which are also called naphthalenediamines. They are usually prepared by reducing the nitronaphthalene derivative and aminating the subsequent hydroxynaphthalene derivative.

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Phenol Red is a phenolsulfonphthalein compound. It is present as a sulfonated hydroxyquinone in weakly acidic aqueous solutions. In alkaline solutions, the dye forms a hydrophilic dianion (a small, red anion and has a larger conjugated system than the yellow hydroxyquinone).

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Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.

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Reichardt′s dye belongs to the class of azo merocyanine dyes. Reichardt′s dye, also known as ET(30) or pyridinium N-phenoxyde betaine dye is a highly solvatochromic betaine dye useful for determining solvent polarity effects. It plays an important role as a probe of solvent polarity in biological systems and on surfaces. Reichardt’s dye, a solvatochromic betaine dye has been used for the determination of solvent polarity.

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Cresol red sodium salt, Sigma-Aldrich®

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Congo red, Sigma-Aldrich®

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Methyl-3-amino-5,6-dichloropyrazine-2-carboxylate 97%, Sigma-Aldrich®

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Standard stain for microscopy. It may be also be used as a reference standard in the determination of azocarmine G in food products and beverages using high performance liquid chromatography coupled with diode array detector (HPLC-DAD).

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A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

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Acridine Orange solution.

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An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

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Reactive Blue 4, Dye content 35 % (1-Amino-4-[3-(4,6-dichlorotriazin-2-ylamino)-4-sulfophenylamino]anthraquinone-2-sulfonic acid; Procion(R) blue MX-R)

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Fluorescent molecular rotor

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Mixture of crystal violet [C.I.No. 42555] and methyl violet [C.I.No. 42535]. Dye employed for preparing Gram stain.

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Sunset yellow FCF is a food dye, which is is substantially used in various food preparations. It is a orange-red coloured crystal, that is soluble in water and ethanol. It is used in treating bone metabolic diseases, dermatological disorders, respiratory illness etc. It can alter the functional responses of splenocytes at non-cytotoxic dose. It promotes immune suppression. Relative expression of surface receptors in T and B cells can be altered by sunset yellow FCF.