"SEC Chromatography"
Gel filtration media, Sephadex™ G-100
Supplier: Cytiva
Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.
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Size exclusion chromatography columns, illustra™ NAP™
Supplier: Cytiva
NAP™ columns are disposable columns prepacked with Sephadex™ G-25 DNA grade and require only gravity to run.
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Pre-packed columns, 1 and 5 ml, SkillPak™
Supplier: TOSOH Bioscience
SkillPak 1 and 5 ml columns are designed for fast method development, resin screening or sample concentration. Pre-packed with TOYOPEARL®, TSKgel® or Ca++Pure-HA™ process chromatography media, these columns offer superior separation of biomelocules, such as monoclonal antibodies, proteins and oligonucleotides. SkillPak 50 and 200 ml pre-packed columns are the best solution for seamless scale up of methods developed on SkillPak 1 and 5.
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Sepharose™ 6B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ 6B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Sepharose™ 4B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Sepharose™ CL-2B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Pierce™ Desalting Columns, Polyacrylamide
Supplier: Thermo Fisher Scientific
Protein desalting spin columns are designed to desalt or exchange buffer of protein samples with volumes of 30 to 120 µl. These devices have exceptional desalting characteristics with ≥95% retention of salts and small molecules while providing excellent recovery of proteins greater than 7 kDa. Multiple samples can be processed in less than five minutes without cumbersome column preparation steps.
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Superose™ 12 Gel Filtration Media, Prep Grade
Supplier: Cytiva
Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability
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Desalting columns, HiPrep™
Supplier: Cytiva
HiPrep™ 26/10 desalting column is a convenient, easy to use column pre-packed with Sephadex™ G-25 Fine for desalting and buffer exchange with sample sizes of up to 15 ml per column.
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Columns, PD SpinTrap™ G-25
Supplier: Cytiva
PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules.
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Gel filtration columns, HiPrep™ with Sephacryl® S-200 HR
Supplier: Cytiva
HiPrep™ 16/60 Sephacryl® S-200 HR and HiPrep™ 26/60 Sephacryl® S-200 HR are pre-packed gel filtration columns for preparative separation of biomolecules of up to 5 and 13 ml sample volume respectively. Sephacryl® S-200 is used for separating mid-size proteins including antibodies and serum proteins.
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Gel filtration columns, HiPrep™ with Sephacryl® S-400 HR
Supplier: Cytiva
HiPrep™ 16/60 Sephacryl® S-400 HR and HiPrep™ 26/60 Sephacryl® S-400 HR are pre-packed gel filtration columns for preparative separation of biomolecules of up to 5 and 13 ml sample volume respectively. Sephacryl® S-400 is used for separating polysaccarides, macromolecules with extended structures, and even small plasmids.
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Gel filtration columns, HiPrep™ with Sephacryl® S-500 HR
Supplier: Cytiva
HiPrep™ 16/60 Sephacryl S-500 HR and HiPrep™ 26/60 Sephacryl® S-500 HR are pre-packed gel filtration columns for preparative separation of biomolecules of up to 5 and 13 ml sample volume respectively. Sephacryl® S-500 is used for separating polysaccarides, macromolecules with extended structures, and even small plasmids.
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Columns, PD MiniTrap™ G-10
Supplier: Cytiva
PD MiniTrap G-10 columns are prepacked, single-use gravity columns for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides.
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Gel filtration calibration kits
Supplier: Cytiva
Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns, such as Superdex, Superose and Sephacryl.
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Desalting columns with Sephadex G-25 resin, HiTrap™
Supplier: Cytiva
HiTrap Desalting are ready-to-use 5 ml columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange.



