74283 Results for: "MP Biomedicals"

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Anti-IgG, C3 IgG Polyclonal Antibody

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Chlortetracycline hydrochloride has antimicrobial action similar to tetracycline hydrochloride, but it is somewhat less active against many Gram-negative organisms. It was first isolated from the culture of Streptomyces aureofacien.

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Isodesmosine is prepared according to the method of Starcher, et al. from hydrolysed bovine neck ligament by solvent extraction and chromatography on Dowex 50. It is chromatographically purified hygroscopic crystalline powder.

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C1q, together with C1r and C1s, in the ratio of 1:2:2, form the C1 complex which is the first component of the classical complement pathway. It is composed of 18 polypeptide chains (six A, six B, six C). All contain an 81 amino acid collagen like region composed of (Gly-Xaa-Yaa) repeating sequences close to the N-terminus. Three chains (A1B1C1) form a triple helix with the C-terminus forming the globular heads which may be structurally and functionally distinct domains.

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RNase ERASE™ decontamination solution has been formulated using three ingredients known to be active against RNase.

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Polynucleotide, carrier of genetic material.

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Storage: Store at Room Temperature (15-30 °C). Store Desiccated.
L-Asparagine is used in cell culture media and is a component of MEM non-essential amino acids solution. L-Asparagine has been shown to enhance ornithine decarboxylase activity in cultured human colon adenocarcinoma Caco-2 cells and in cultured IEC-6 intestinal epithelial cells. Spore germination in Bacillus subtilis has been increased in the presence of L-asparagine. An isoxazoline RGD mimic platelet GPIIb/IIIa antagonist has been prepared by chiral synthesis with L-asparagine as a starting material. L-Asparagine has been utilized in the synthesis of 4-azalysine building blocks for application to combinatorial chemistry.

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Cefsulodin sodium salt

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Cholesterol is the principal sterol of the higher animals, found in all body tissues. It is the main constituent of gallstones and all biological membranes. Approximately 25% of total brain lipid is cholesterol.

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D-(+)-Glucose is ideal as a culture media component.

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Thiamine hydrochloride, powder

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Product is rabbit IgG (whole molecule) covalently coupled to Sepharose-4B, and packed in a polystyrene chromatography column.IgG affinity gel contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because this gel is not a bind-and-release support, it is extremely fast and gentle to antibodies, resulting in antibody preparations of high purity and high activity. IgG affinity gel works with antibodies from a variety of species and subclasses, many of which do not purify efficiently with Protein A or Protein G. The resulting recovery and purity of the IgG isolated by this method rivals that obtained from the same samples using bind-and-release supports such as Protein A or Protein G. Furthermore, antibodies purified with this gel are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralize or desalt the recovered antibody to remove harsh elution buffers.

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Adenosine-5'-diphosphate monosodium salt (from equine muscle) ≥97%, powder

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The complement system provides innate defense against microbial infection and is a "complement" to antibody mediated immunity. The complement system consists of thirty five interacting plasma and membrane associated proteins which contribute to host-defense and initiate and amplify inflammation, even in the preimmune state where specific antibodies and lymphocytes are not available. In addition to the complement components themselves, this system also contains several soluble factors that prevent spontaneous complement activation from occurring in solution, as well as, several regulatory membrane associated proteins that protect host cells from accidental complement attack. Cleavage of the C3 component by either the classical pathway or the alternative pathway releases C3a and C3b. C3a appears to be important in many inflammatory responses while the C3b fragment covalently binds to the cell or bacterial surface and plays a role in opsonisation. Binding of C3b to the C4b component of the C3 convertase, results in C5 convertase (C4b3b2a) formation

The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected.

This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. The product has been successfully used in Immunoblotting. It detects C3 from human, equine, hamster, Guinea pig, murine, rabbit, rat, pig, and non-human primate samples. It does not cross react wtih bovine, canine, chicken, feline and sheep samples. It has been successfully used in immunocytochemistry, Western blot, Immunoelectrophoresis, and flow cytometry applications. It detects C3 from human, equine, hamster, Guinea pig, murine, rabbit, rat, pig, and non-human primate samples. It does not cross react wtih bovine, canine, chicken, feline and sheep samples. It has been successfully used in immunocytochemistry, Western blot, Immunoelectrophoresis, and flow cytometry applications.

Supplier: MP Biomedicals

Based on immunoelectrophoresis, these antibodies react with the heavy chains on human IgG but not with the light chains on most human immunoglobulins. No antibody was detected against human IgM or IgA or non-immunoglobulin serum proteins, but antibodies may cross-react with immunoglobulins from other species.

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Hemoglobin is the major component of red blood cells, and is responsible for their red color. A major oxygen-transporting component of red blood cells.

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N-(1-Naphthyl)ethylenediamine, dihydrochoride is used for colorimetric determination of sulfonamide in blood. It is used for spectrophotometric determiniation of nitrite and nitrate.

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Anti-beta-galactosidase Goat Polyclonal Antibody

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Dextran is a high molecular weight polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(1-->6) linkages. Dextrans with molecular weights greater than 10,000 behave as if they are highly branched. As the molecular weight increases, dextran molecules attain greater symmetry.

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Deoxycytidine is a deoxyribonucleoside, a component of deoxyribonucleic acid. It is similar to the ribonucleoside cytidine, but with one hydroxyl group removed from the 2' position.

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L-Lysine is an essential proteinogenic α amino acid. It contains an N-butyl amino group in the side chain, and this moiety is protonated at physiological pH. In addition, L-lysine is one of the two amino acids that are degraded to give ketone bodies (a ketogenic amino acid).

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Gentamicin is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M. echinospora. It inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms.

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This serum is produced from blood collected from newly born calves between 1 to 14 days of age.

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Cellulase Y-C removes cell walls from plant tissues in shorter incubation period without loss of biological activity of the materials.

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Pectolyase Y-23 contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. It includes maceration stimulating factor which stimulates tissue maceration by both pectinases.

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Coomassie Brilliant Blue G-250 is a protein stain in electrophoresis. Brilliant Blue G has been used in the Bradford dye-binding protein assay.

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The product contains vitamin A acetate (approximately 21%) finely dispersed in a starch-coated matrix of gelatin (approximately 60%), sucrose (approximately 14%) and corn starch (approximately 5%). BHT and BHA are added as antioxidants.

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Elastase is prepared from porcine pancreas. It is lyophilised and water soluble. Elastase is chromatographically prepared by the method of Narayanan and Anwar. In the method, 2 times crystalline elastase is adsorbed on a column of DEAE-Sephadex A50 to separate elastase and non-specific protein components. The elastase component is further purified by chromatography on a column of CM-Cellulose using a sodium chloride gradient to elute the elastase. The latter is dialysed until chloride-free and then lyophilised. During its preparation the elastase is held below a pH of 5,5 for greater than 24 hours. Two times crystallised from the euglobin fraction of porcine pancreas by the method of Lewis et al. Does not contain trypsin or chymotrypsin.

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Cetyltrimethylammonium bromide is a phase transfer catalyst ideal for the precipitation of DNA. Hexadecyltrimethylammonium bromide is a bactericidal, cationic detergent. Its activity is neutralized by soaps and anionic detergents (i.e., SDS). Trimethylammonium bromide compounds form insoluble complexes with SDS. It is active at alkaline pH against both Gram positive and Gram negative organisms.

Cetyltrimethylammonium bromide ia used as an cationic surfactant suitable for the isolation of high molecular weight DNA in plants and other organisms(by a rapid method) as well as plant DNA for use in PCR analysis. It has been used to precipitate nucleic acids. CTAB provides enhancement of Concanavalin A mediated agglutination. It has been used for the determination of protein molecular weights in electrophoretic systems and for determination of critical micelle concentration of detergents. CTAB has also been used as a titrant for potentiometric titration of perchlorate and as a phase-transfer catalyst in reduction of arenes and heterocyclic compounds.

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BioRich 1 is a DMEM/F-12 powdered media blend enriched with trace elements. This permits the transition to 'serum-free' conditions reducing or eliminating the necessity for serum supplementation.