"MP Biomedicals"

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Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Supplier: MP Biomedicals

5-Fluorouracil (5-FU) inhibits the activity of thymidylate synthetase, which affects pyrimidine synthesis and leads to depletion of intracellular TTP pools.

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Aquaphenol™ is a solution of double-distilled phenol saturated with ultra-pure water (approx 30%). It does not contain antioxidant or additives. Aquaphenol™ has an acid pH and can be used directly for RNA extraction. For DNA extraction, pH can be quickly adjusted to 7,5 or 8 using pH maker solutions and protocols included.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

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L-Asparagine is used in cell culture media and is a component of MEM non-essential amino acids solution. L-Asparagine has been shown to enhance ornithine decarboxylase activity in cultured human colon adenocarcinoma Caco-2 cells and in cultured IEC-6 intestinal epithelial cells. Spore germination in Bacillus subtilis has been increased in the presence of L-asparagine.

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Major electron acceptor molecule in biological oxidations. Acts as a coenzyme for hydride-transfer enzymes and a substrate for NAD(+)-consuming enzymes, which include ADP-ribose transferases, poly(ADP-ribose) polymerases, cADP-ribose synthases and sirtuins. Recently reported to be a P2Y11 agonist.

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Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein.

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Magnesium chloride hexahydrate (MgCl₂·6H₂O) is an inorganic salt that can be generated from the acid leach solution of magnesite tailings. It is a potential phase change material (PCM) for storing thermal energy in solar cookers. The result of altering poly (vinyl alcohol)(PVA) films with MgCl₂·6H₂O has been investigated. The ability of MgCl₂·6H₂O to reduce the flammability of cotton fabrics has been studied.

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Moderately soluble in water. Soluble in methanol(1 mg/ml): yellow to yellow-green solution.

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Glutathione suppresses human immunodeficiency virus expression in chronically infected monocytic cells. It is a useful tripeptide involved in many aspects of metabolism, including transport of g-glutamyl amino acids and reductive cleavage of disulphide bonds.

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A monochlorotriazine dye that inhibits phosphotransferase acitvity

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Coomassie Brilliant Blue G-250 is a protein stain in electrophoresis. Brilliant Blue G has been used in the Bradford dye-binding protein assay.

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Solvent Blue 35 is a member of the Sudan family of hydrophobic fat-staining dyes. In structural classification it is a diazo dyes.
Solvent Blue 35 is predominantly used for staining triglycerides in animal tissues (frozen sections). With the use of certain solvents, may also be used to stain some protein bound lipids in paraffin sections.

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Inhibitors: p-Chloromercuribenzoate, iodoacetamide, heavy metal ions (Zn²⁺, Fe²⁺, Zn²⁺, Cd²⁺, Cu²⁺, Pb²⁺, Ag⁺, Hg²⁺), ionic Detergents (SDS, DAC, etc.). Contaminants: The preparation is practically free from other glycosidases (a-galactosidase, a-,b-glucosidase, a-,b-mannosidase, etc.) and proteinase. Principle: o-Nitrophenyl-b-D-galactopyranoside (ONPG) b-galactosidase > o-Nitrophenol (ONP) + D-Galactose. The appearance of o-nitrophenol is measured at 410 nm by spectrophotemetry. Thermal Stability: Below 50 °C (pH 7,3; 15 min) (Lit.), Optimum Temperature: 50 to 55 °C (Lit.).

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Sodium acetate is used as a buffer in the buffering range of pH 3.6-5.6. It is also used in the purification and precipitation of nucleic acids, protein crystallization, staining of gels in protein gel electrophoresis, HPLC, mordant dyeing, DNA microarray studies of E. coli response, investigation of protein unfolding during reverse phase chromatography.

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Olive oil is commonly used in cooking, cosmetics, pharmaceuticals, and soaps and as a fuel for traditional oil lamps.