"GeCKO Libraries"

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Lentiviral Control Particles

Supplier: OriGene

Lenti particles carrying a 29-mer scrambled sequence cassette and serve as a negative control.

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Human Tagged ORF Clone Lentiviral Particle, CD252 (TNFSF4)

Supplier: OriGene

7 TU/ml.

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Mouse Tagged ORF Clone, Basp1

Supplier: OriGene

Basp1 mouse tagged ORF clone expression plasmid. Basp1 (Myc-DDK-tagged) - Mouse brain abundant, membrane attached signal protein 1 (Basp1).

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Lambda DNA

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Lambda DNA is a temperate E. coli bacteriophage.

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Mammalian TAP system, interPlay

Supplier: AGILENT

The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.

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pAAV-AC-GFP AAV Gene Expression Vector

Supplier: OriGene

AAV Gene Expression Vectors with C-terminal tGFP tag.

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Mouse Untagged Clone Plasmid, Lrrc15 (NM_028973)

Supplier: OriGene

Lrrc15 (untagged) - Mouse leucine rich repeat containing 15 (Lrrc15).

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E. coli BL21

Supplier: Cytiva

The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.

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pET-14b DNA, Novagen®

Supplier: Merck Millipore (Novagen)

The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.

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The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

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pET-30a(+) DNA, Novagen®

Supplier: Merck Millipore (Novagen)

The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.

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Expression vectors, pET-41 DNA, Novagen®

Supplier: Merck Millipore (Novagen)

pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

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Cell-free protein synthesis, rapid translation system (RTS) vectors

Supplier: biotechrabbit

Expression vectors for PCR-based generation of linear templates.

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Cell-free protein expression vectors, T7, mammalian

Supplier: Thermo Fisher Scientific

The T7 cell-free expression vectors (pT7CFE1) are cloning plasmids optimised for use with the Thermo Scientific™ 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation.