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"GeCKO Libraries"

 
 

Lentiviral Control Particles

Supplier: OriGene

Lenti particles carrying a 29-mer scrambled sequence cassette and serve as a negative control.

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InterPlay adenoviral TAP system

InterPlay adenoviral TAP system

Supplier: AGILENT

The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.

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Human Tagged ORF Clone, Androgen Receptor (AR) (NM_000044)

Supplier: OriGene

The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10 µg of transfection-ready, dried plasmid DNA (reconstitute with 100 µl of water).

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sparQ mRNA-Seq Kit

sparQ mRNA-Seq Kit

Supplier: Quantabio

Ultra-sensitive mRNA library prep workflow with efficient poly(A) capture.

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Human Tagged ORF Clone, SPCA2 (ATP2C2)

Supplier: OriGene

ATP2C2 (tGFP-tagged) - Human ATPase, Ca++ transporting, type 2C, member 2 (ATP2C2), transcript variant 3.

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ORF Clones, GABA B receptor 1 (GABBR1), human tagged, TrueORF®

Supplier: OriGene

GABA B receptor 1 (GABBR1), human tagged ORF clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Untagged FBXL17 Clone

Supplier: OriGene

FBXL17 (untagged)-Human F-box and leucine-rich repeat protein 17 (FBXL17). The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10 µg of transfection-ready, dried plasmid DNA (reconstitute with 100 µl of water).

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Expression vectors, pGEX

Supplier: Cytiva

The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

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pET-14b DNA, Novagen®

Supplier: Merck Millipore (Novagen)

The pET-14b vector carries an N-terminal His·Tag® sequence followed by a thrombin cleavage site and three cloning sites.

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E. coli BL21

E. coli BL21

Supplier: Cytiva

The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.

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M13KO7 Helper Phage, Cytiva

M13KO7 Helper Phage, Cytiva

Supplier: Cytiva

M13 phage for the GST expression system.

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Vector, pET-24d(+) DNA

Supplier: Merck Millipore (Novagen)

The pET-24a-d(+) vectors carry an N-terminal T7·Tag® sequence plus an optional C-terminal His·Tag® sequence.

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Vector, pET-24(+) DNA

Supplier: Merck Millipore (Novagen)

pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His·Tag® sequence is available

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Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors

Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors

Supplier: Thermo Fisher Scientific

Gaussia Dura Luc vectors contain a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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Expression vectors, green renilla Luc vector for luciferase assays, Pierce™

Expression vectors, green renilla Luc vector for luciferase assays, Pierce™

Supplier: Thermo Fisher Scientific

Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis

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Slc7a11 (NM_011990) mouse tagged ORF clone

Supplier: OriGene

Slc7a11 (GFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter y+ system) member 11 (Slc7a11), (10 µg).

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