28463 Results for: "G-Biosciences"

Supplier: G-Biosciences

G-Biosciences offers 0.9% NaCl in two convenient sizes to meet your particular laboratory needs. These sterile saline solutions are suitable for numerous applications.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: G-Biosciences

Sodium azide 1% in aqueous solution

Supplier: G-Biosciences

Laboratory reagent grade, sterile distilled water for your laboratory needs. This water is purified by steam distillation and sterile filtered for use as a research laboratory reagent. The distilled water is low endotoxin <0.025EU/ml and low conductivity (<10uS). This water is ideal for the preparation of reagents, rinsing of glassware and plasticware, and various other laboratory and research applications. No toxic agents such as DEPC are included in the preparation of distilled water, eliminating interference with enzymatic analysis.

Supplier: G-Biosciences

PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

Supplier: G-Biosciences

Molecular Grade Water is ultrapure water that certified RNase, DNase and Protease free, is suitable for use in molecular biology applications, which demand a high quality of water and assurance that the water is free from nucleases and protease contamination. No toxic agents, such as DEPC, are used in the manufacturing of Molecular Grade Water, eliminating DEPC interference of enzymatic reactions.

Supplier: G-Biosciences

LongLife™ Zymolyase®, 1,5 U/µl

Supplier: G-Biosciences

These empty columns are designed for fast protein liquid chromatography.

Supplier: G-Biosciences

10X PBS (Phosphate Buffered Saline) is a 10 fold concentrated, sterile, pH adjusted blend of phosphate buffer and saline solution, which when diluted 1 to 10 in ultrapure water produces a phosphate buffered saline of 8mM NaH2PO4, 150mM NaCl, 3mM KCl, and 2mM KH2PO4 at pH 7.4.

Supplier: G-Biosciences

The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

Supplier: G-Biosciences

The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.

Supplier: G-Biosciences

BT Lab systems horizontal gel electrophoresis set's abundant buffer not only guarantees cooling effects, but also keeps the pH value stable during operation.

Supplier: G-Biosciences

G-Trap™ GT desalting columns are prepacked ready to use columns for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography. Mostly used for removal of salt or buffer exchange before or after different chromatographic steps. They can also be used for separation of proteins based on their sizes.

Supplier: G-Biosciences

HOOK™ Sulpho-NHS-SS-Biotin, 50 mg

Supplier: G-Biosciences

There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.

Supplier: G-Biosciences

G-Biosciences' Coomassie Brilliant Blue (CBB) G-250 and R-250 Stain are based on a colloidal Coomassie stain. CBB G-250 and R-250 stain proteins with high band visibility. The staining of gels with CBB G-250 and R-250 allows the examination of protein bands even during the staining process.

Supplier: G-Biosciences

PIPES monosodium salt

Supplier: G-Biosciences

γ-(N-Morpholino)propanesulphonic acid (MOPS)

Supplier: G-Biosciences

Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

Supplier: G-Biosciences

N-Decyl-N,N-dimethyl-3-ammonio-1-propanesulphonate(sulphobetaine-10, SB-10)

Supplier: G-Biosciences

D-(-)-Leucine

Supplier: G-Biosciences

Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.

Supplier: G-Biosciences

HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.

Supplier: G-Biosciences

Sodium hydroxide

Supplier: G-Biosciences

The Apoptotic DNA Ladder Kit contains the reagents necessary for the preparation of cellular fractions for the production of apoptosis nucleosomal DNA ladders. The kit does not require toxic phenol and DNA ladder is prepared either with or without genomic DNA.

Supplier: G-Biosciences

Running Buffer, Tris-Glycine [10X]

Supplier: G-Biosciences

Magnesium chloride

Supplier: G-Biosciences

ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

Supplier: G-Biosciences

FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. Complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions in traditional silver staining kits inhibit proteolytic digestion. In addition, glutaraldehyde, a common sensitiser in silver stains, modifies peptide lysine residues preventing complete digestion and recovery.

Supplier: G-Biosciences

Disodium hydrogen phosphate