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28466 Results for: "G-Biosciences"

Buffer, TBS solution 10X concentrate (TRIS buffered saline)

Supplier: G-Biosciences

The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

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Sodium azide 1% in aqueous solution

Supplier: G-Biosciences

Sodium azide 1% in aqueous solution

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Pfu DNA polymerase

Pfu DNA polymerase

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

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Water, sterile, distilled

Supplier: G-Biosciences

Laboratory reagent grade, sterile distilled water for your laboratory needs. This water is purified by steam distillation and sterile filtered for use as a research laboratory reagent. The distilled water is low endotoxin <0.025EU/ml and low conductivity (<10uS). This water is ideal for the preparation of reagents, rinsing of glassware and plasticware, and various other laboratory and research applications. No toxic agents such as DEPC are included in the preparation of distilled water, eliminating interference with enzymatic analysis.

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Phosphate buffered saline (PBS), 10X

Phosphate buffered saline (PBS), 10X

Supplier: G-Biosciences

PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

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Water, Molecular Grade DNase-, RNase-free

Water, Molecular Grade DNase-, RNase-free

Supplier: G-Biosciences

Molecular Grade Water is ultrapure water that certified RNase, DNase and Protease free, is suitable for use in molecular biology applications, which demand a high quality of water and assurance that the water is free from nucleases and protease contamination. No toxic agents, such as DEPC, are used in the manufacturing of Molecular Grade Water, eliminating DEPC interference of enzymatic reactions.

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Accessories for protein kits

Accessories for protein kits

Supplier: G-Biosciences

LongLife™ Zymolyase®, 1,5 U/µl

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Experimentation kits, bacterial gram staining

Experimentation kits, bacterial gram staining

Supplier: G-Biosciences

The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.

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Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: G-Biosciences

10X PBS (Phosphate Buffered Saline) is a 10 fold concentrated, sterile, pH adjusted blend of phosphate buffer and saline solution, which when diluted 1 to 10 in ultrapure water produces a phosphate buffered saline of 8mM NaH2PO4, 150mM NaCl, 3mM KCl, and 2mM KH2PO4 at pH 7.4.

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Empty FPLC columns, G-TRAP™

Empty FPLC columns, G-TRAP™

Supplier: G-Biosciences

These empty columns are designed for fast protein liquid chromatography.

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Sodium chloride 0.9% in water normal saline solution

Supplier: G-Biosciences

G-Biosciences offers 0.9% NaCl in two convenient sizes to meet your particular laboratory needs. These sterile saline solutions are suitable for numerous applications.

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Immobilised Catalase

Supplier: G-Biosciences

Immobilised Catalase is prepared by covalent coupling of catalase enzyme to 6% cross-linked agarose beads. Immobilised Catalase is used to remove hydrogen peroxide from biological samples for use in downstream applications which require absenceof hydrogen peroxide as it causes interference.

 

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Pig Native Gelatin (from Skin)

Supplier: G-Biosciences

Pig Native Gelatin (from Skin)

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Recombinant protein purification resins, IMAC

Recombinant protein purification resins, IMAC

Supplier: G-Biosciences

Cobalt chelating resin, for the purification of 6X His proteins

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picoLUCENT™ PLUS-HRP Kit

picoLUCENT™ PLUS-HRP Kit

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

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Immunodetection reagents, femtoLUCENT™ PLUS

Immunodetection reagents, femtoLUCENT™ PLUS

Supplier: G-Biosciences

The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

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DNA ladder, 100 bp Plus, DNAmark™

DNA ladder, 100 bp Plus, DNAmark™

Supplier: G-Biosciences

DNAmark™ 100 bp Plus ladder consists of 14 DNA fragments ranging in size from 100 to 3000 base pairs (bp). The 500 bp and 1200 bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500 bp and 1200 bp are 40 ng. The 500 bp and 1200 bp fragment are 100 ng.

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DNA ladder, 100 bp, DNAmark™

DNA ladder, 100 bp, DNAmark™

Supplier: G-Biosciences

DNAmark™ 100 bp ladder consists of 11 fragments ranging from 100 to 1500 base pairs (bp). The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500 bp are 40 ng. The 500 bp fragment is 100 ng.

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TRITON™ X-100 (Polyethylene glycol tert-octylphenyl ether) 10% in water

Supplier: G-Biosciences

TRITON™ X-100 (Polyethylene glycol tert-octylphenyl ether) 10% in water

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Magnetic stand

Magnetic stand

Supplier: G-Biosciences

Magnetic stand is designed for the efficent pull down of magnetic beads in 1,5 and 2 ml micro centrifgue tubes.

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X-GlcA ((5-bromo-4-chloro-3-indolyl-ß-D-glucuronide) cyclohexylammonium salt)

Supplier: G-Biosciences

X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid) is a substrate for ß-glucuronidase (GUS), which is encoded by gusA, a widely used reporter gene. The glucuronidase cleaves X-Gluc to produce colourless glucuronic acid and an intense blue precipitate of chloro-bromoindigo. X-Gluc is used for the histochemical detection of GUS expression in plant cells and tissues, detection of infections caused by E.coli and detection of bacterial contamination in food and water samples.

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ELISA chemiluminescent detection system, OptiBlaze™

ELISA chemiluminescent detection system, OptiBlaze™

Supplier: G-Biosciences

OptiBlaze™ ELISA femto-HRP and femto-AP are stabilised ultra sensitive luminol and 1,2 dioxetane based horseradish peroxidase or alkaline phosphatase substrates for the detection of HRP or AP-conjugated antibodies during ELISA.

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Kidney tissue region blot, single species

Kidney tissue region blot, single species

Supplier: G-Biosciences

These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.

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Gravity flow columns

Gravity flow columns

Supplier: G-Biosciences

Column type: Flash/LPLC column, Gravity flow column, total volume 32 ml, resin volume 20 ml, reservoir volume 12 ml, graduated, with screw cap (frit 3 mm ~30 µm PE), Empty columns: Yes, Capacity: 20 ml

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Affinity purification resins, Glutathione Resin

Affinity purification resins, Glutathione Resin

Supplier: G-Biosciences

Glutathione resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8 mg GST/ml resin. Supplied as slurry in 20% ethanol.

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Protein fractionation kit, FOCUS™ Membrane Proteins

Protein fractionation kit, FOCUS™ Membrane Proteins

Supplier: G-Biosciences

FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.

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FOCUS™ Yeast proteome kits

FOCUS™ Yeast proteome kits

Supplier: G-Biosciences

Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

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SilverOUT™, G-Biosciences

SilverOUT™, G-Biosciences

Supplier: G-Biosciences

SilverOUT removes interfering silver ions from gel pieces.

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Protein precipitation and concentration kit, UPPA-PROTEIN-Concentrate™

Protein precipitation and concentration kit, UPPA-PROTEIN-Concentrate™

Supplier: G-Biosciences

UPPA PROTEIN-Concentrate™ Kit uses a proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentrate dilute protein samples as low as 1 ng/ml. Concentration is not affected by the presence of common laboratory agents, including detergents and chaotropes. After precipitation, the sample is washed to remove salts and other interfering agents; complete recovery of sample is produced.

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Biotin conjugation estimation, HOOK™ BiotinQuant

Supplier: G-Biosciences

HOOK™ BiotinQuant is designed for the estimation of biotin conjugation. It measures biotin using HABA [4’-hydroxyazobenzene-2-carboxylic acid] dye. HABA binds with avidin at the biotin-binding site.

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