328 Results for: "Electrophoresis Buffers"

Supplier: Thermo Fisher Scientific

Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.

Supplier: Cytiva

DIGE gel is a 12,5% precast polyacrylamide gel in a low fluorescent glass cassette for second dimension 2-D electrophoresis and specially developed for 2-D DIGE analysis. DIGE gel and DIGE buffer kit are tools to increase the quality, reproducibility, and convenience of 2-D DIGE using Ettan™ DIGE system.

Supplier: G-Biosciences

Kit Protein Folding Study includes PAGE mix (19:1 acrylamide/bisacrylamide), ammonium persulfate (APS), detergent solution (10% SDS) , PAGE stacking buffer, PAGE separating buffer, TEMED, sample loading buffer (2X concentrated), electrophoresis runni 1 * 1 KIT

Supplier: Cytiva

The key to outstanding, reproducible electrophoresis results is temperature control. Gels unequally cooled on their two faces produce slanted bands appearing as broad or double bands in stained gels. SE 600 Ruby™ ensures uniform heat in gels because, firstly, the gels are completely immersed in the lower buffer for uniform heat transfer and, secondly, a standard magnetic stirrer circulates buffer around the vertically suspended heat exchanger to maintain homogeneous lower buffer temperature. The ergonomically designed SE 600 Ruby™ provides two built-in side handles, which facilitates carrying and handling.

Supplier: Genscript

GenScript's Bis-Tris gels are high-performing polyacrylamide gels designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimises polyacrylamide hydrolysis and increases gel stability. Furthermore, the neutral pH which minimises protein modification when compared to Tris-glycine gels.

Supplier: VWR Collection

The shiroGEL vertical 20 offers application flexibility and is distinguished by its ease of gel casting and set-up, requiring only four screws to secure up to four 20×20 cm gels. This vertical screw clamp technology distributes pressure evenly along the height of gel rather than in the centre to eliminate plate bowing and gel compression, while maintaining a leakproof seal during casting. A built-in inner buffer chamber within the PAGE insert allows set up to be completed without inclusion of an upper buffer chamber. These systems are ideal for 1D and 2D SDS-PAGE, native, preparative, gradient and high resolution nucleic acid electrophoresis.

Supplier: Biotium

DNA ladders in TE buffer are used for sizing linear double-stranded DNA fragments separated by gel electrophoresis. The 1 kb DNA ladder is suitable for sizing DNA fragments from 250 bp to 10 kb, while the 100 bp DNA ladder is suitable for sizing DNA fragments from 100 bp to 1500 bp.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15 - 30 °C)
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. Glycerol is used in the concentration and storage of enzymes. Also prevents back-diffusion and protein samples into the buffer. Ideal for use as a cryoprotectant.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Supplier: EMBITEC

The ultra compact RunOne™ electrophoresis system is able to run a whole variety of gel sizes from mini to long in less than 350 ml of buffer. The power supply with selectable 25, 50 or 100 V output slides directly into the running unit. The system can run eight to 108 samples in minutes, with the separate casting system allowing up to six gels to be cast and run at the same time.

Supplier: Biotium

Go-Go Fast DNA Gel Running Buffer is a high voltage running buffer for agarose gel electrophoresis. With Go-Go Fast, you can run DNA gels up to 300V for 3X faster run times compared to TBE or TAE gels and superior DNA resolution.500 mL of 50X buffer 1 * 500 mL

Supplier: Biotium

Go-Go Fast DNA Gel Running Buffer is a high voltage running buffer for agarose gel electrophoresis. With Go-Go Fast, you can run DNA gels up to 300V for 3X faster run times compared to TBE or TAE gels and superior DNA resolution. 200 mL of 50X buffer 1 * 200 mL

Supplier: MP Biomedicals

Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). Tris does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is an auxiliary material in pharmaceutical science.
Store at Room Temperature (15-30 °C). Store dessicated.

Supplier: MP Biomedicals

Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is RNAse and DNAse-free. Tris is relatively non-hygroscopic ; but, if needed, it can be dried at 100 °C for up to 4 hours to remove any water.
Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.

Supplier: VWR Collection

Designed for ease of use and safe operation, the shiroGEL horizontal electrophoresis systems and accessories are built to withstand the rigours of everyday use. For leakproof performance the gel boxes and gel trays are moulded from thick acrylic. UV transparent gel trays aid in visualisation of samples. Cassettes protect the electrodes and allow for easy replacement. Gel casting is simple with the durable rubber casting gates. Slots in the sides of the trays allow for easy comb placement. The two-piece design of the combs allows for vertical adjustment, giving the user control over the depth of the well. The combs supplied with the systems are 1,5 mm thick. Multiple combs, including those compatible with multichannel pipettes and different thicknesses, are also available. The 'EasyLift' gel box lid is easily removed using the side tabs and pressure pads. The lid is domed, to prevent condensation from dripping onto the gel, and is surrounded by a drip ring, to help recover the condensate and maintain buffer concentration.

Supplier: Thermo Fisher Scientific

Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Supplier: MP Biomedicals

This product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. Specificity for human IgG is determined by immunoelectrophoresis (IEP) versus purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins.Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation in the gamma region.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: VWR Chemicals

EZ-Vision DNA dye as loading buffer provides a non mutagenic, environmentally friendly alternative to ethidium bromide for instantaneous fluorescent DNA band visualisation.

Supplier: VWR Chemicals

This loading dye base is used for custom loading buffer preparation. It is a concentrated solution containing patent blue, bromophenol blue and amaranth tracking dyes, which migrate at approximately 4 000, 400 and 10 bp, on a 1% agarose gel, respectively.

Supplier: Biotium

6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.

Supplier: VWR Chemicals

These DNA loading dyes are used to load DNA samples to agarose or SDS DNA gels for gel electrophoresis.

Supplier: VWR Collection

The shiroGEL mini vertical PAGE system is modular, allowing PAGE and electroblotting to be carried out in the same tank, simply by changing the inserts. The system features inserts for each application, and a common buffer tank in which to run them. Each insert has its own electrode assembly, which connects to the lid of the buffer tank. For safety, the power leads connect directly to the lid. When the lid is removed, power is disconnected from the system. The inserts also have a small pad that fits into position in the lid. This ensures that the lid is always properly placed and aids in its removal. The buffer tank is moulded to prevent separation and leaking. A variety of spacers, combs and other accessories are available to customise the system. All spacers and combs are colour coded or clearly labelled to indicate thickness.

Supplier: Thermo Fisher Scientific

6X DNA Loading Buffer is a pre-mixed solution used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels.

Supplier: THERMO OWL SCIENTIFIC

The Owl™ B2 EasyCast™ mini horizontal gel system provides flexibility in a small footprint and is available with or without buffer exchange ports. The Owl™ B3 with built-in recirculation system prevents formation of pH and ionic gradients for high resolution and uniform reproducible results. The Owl™ B3 is ideal for long runs, multiple sample sets or RNA gels. It also eliminates uneven migration, band distortion or disassociation of pH dependent glycoxylated RNA molecules that can result when ionic depletion occurs. As the recirculation system is built into the buffer chamber, no external pumps, tubing or stirring bars are required.

Supplier: LONZA

Formaldehyde sample buffer, For: FlashGel® system

Supplier: THERMO OWL SCIENTIFIC

The Owl™ B1A EasyCast™ mini gel system is designed to provide flat, even banding patterns and consistent results with minimum effort. This system is ideal for determination of molecular weight ranges of PCR fragments or larger sized DNA molecules. The all-in-one design allows the option of casting and running a gel in the buffer chamber, eliminating the need for additional equipment.

Supplier: G-Biosciences

Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimise reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on 2D gels.

Supplier: EDVOTEK

0.8% UltraSpec-Agarose™ prepared with TAE buffer. Simply melt, cool and pour.

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.