341 Results for: "EDVOTEK"

Supplier: EDVOTEK

A set of coloured beads that can be designated to represent the Watson-Crick DNA bases: A, T, G, C.

Supplier: EDVOTEK

Proteinase K

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This experiment uses PCR to amplify a small section of Lambda DNA via a 2-step process, saving valuable classroom time and allowing completion of the lab in one session.

Supplier: EDVOTEK

Electrophoresis Gels, UltraSpec-Agarose™

Supplier: EDVOTEK

Capable of running one or two groups of student samples at the same time and producing excellent results in 30 to 40 minutes.

Supplier: EDVOTEK

In this experiment, total protein extracts from several bacterial sources are extracted and compared. The unique patterns of protein bands, obtained by SDS vertical polyacrylamide electrophoresis, can be used to identify various bacterial strains.

Supplier: EDVOTEK

Horizontal gel electrophoresis system

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Water baths incorporating digital temperature control and an optional shaking capacity (10 L model only).

Supplier: EDVOTEK

This lightweight, cordless pipetting controller is ideally suited as an aliquoting tool for instructors and teaching assistants. It uses all standard serological pipettes. The speed can be fine-tuned by applying varying finger pressure to the operating buttons.

Supplier: EDVOTEK

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.

Supplier: EDVOTEK

FlashBlue™ is a DNA stain that offers simple and rapid staining of agarose gels.

Supplier: EDVOTEK

When normal cells are grown in culture they stop growing when they become overcrowded (contact inhibition). Cancer cells in culture grow in an uncontrolled way because they have lost this property. This helps tumours to form in the body. In addition, many different cell types can be present in a single tumour. This experiment allows students to see the differences between normal and cancer cells in both their growth and cell types.

Supplier: EDVOTEK

Oil spills cause devastation to the environment, killing sea life, birds, and coastal plants. Spraying areas of contamination with oil-eating microbes accelerates the degradation of the oil. This process is known as bioremediation. In this open-ended experiment, students grow a mixture of oil-eating bacteria and observe their effectiveness at degrading a variety of oils.

Supplier: EDVOTEK

When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.

Supplier: EDVOTEK

Cancer cells differ from normal cells by the combinations of proteins that are present on their surfaces. Antibodies against these proteins will specifically bind to cancer cells and not to normal cells. This allows early detection of cancer and potentially a way of delivering cancer therapies. In this simulation experiment the reaction of cancer cell markers and their corresponding antigens are demonstrated.

Supplier: EDVOTEK

This experiment introduces students to the use of DNA fingerprinting in a simulated paternity determination. A child's DNA fingerprint is compared with his parents.

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Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.

Supplier: EDVOTEK

According to the World Health Organisation, between two and three million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.

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Educational Materials, Microbiology, Application: Microbiology

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The body defends itself from attack by infectious agents like bacteria and viruses by producing antibodies. Enzyme linked immunosorbent assays (ELISA) test for antibodies present in the blood, which indicate infection. In this experiment, students perform a simulated ELISA test to identify infected samples and compare them to control samples.

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X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)

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Glass thermometer

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Experimentation kits, electrophoretic properties of native proteins (agarose-based)

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Restriction enzyme reaction buffer

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Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.

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Scents and sense-ability kit

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Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.

Supplier: EDVOTEK

The three most frequently used restriction enzymes are Eco RI, Bam HI and Hind III. Each enzyme catalyses cleavage at the defined base sequence. All enzymes are lyophilised and contain 1500 units. One unit is defined as the amount of enzyme required to digest 1,0 µg of lambda DNA in 60 minutes at 37 °C in a total reaction mixture of 50 µl.

Supplier: EDVOTEK

Evidence left behind at a crime scene can identify a potential culprit. Even in this age of DNA, fingerprints and blood stains are still important in helping to identify a criminal. In this experiment, students solve a crime by dusting for fingerprints and use fluorescent dust to search for and identify trace amounts of blood.

Supplier: EDVOTEK

In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members.