"Cytiva"
Amersham™ Protran® Western Blotting Membranes, Nitrocellulose
Supplier: Cytiva
Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.
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Dye terminator removal, illustra™ AutoSeq™ G-50 columns
Supplier: Cytiva
illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.
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Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow
Supplier: Cytiva
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
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Affinity chromatography columns, HiTrap™ Protein A HP
Supplier: Cytiva
HiTrap™ Protein A HP is a pre-packed, ready to use Protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum and cell culture supernatants.
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Sepharose™ CL-2B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media
Supplier: Cytiva
Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.
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Columns, PD MiniTrap™ G-25
Supplier: Cytiva
PD MiniTrap G-25 columns are prepacked, single-use gravity columns for desalting, buffer exchange, and sample cleanup.
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HiLoad Superdex columns, 75 PG
Supplier: Cytiva
Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component
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Affinity coupling media, CNBr-Activated Sepharose™ 4B
Supplier: Cytiva
CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
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Protease affinity chromatography media, PreScission™, Cytiva
Supplier: Cytiva
Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.
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Ion exchange chromatography media, IEX Sepharose™ Fast Flow
Supplier: Cytiva
Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades.
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Affinity chromatography columns, HisTrap™ HP
Supplier: Cytiva
HisTrap HP prepacked columns provide a fast, convenient and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.
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Affinity chromatography columns, HiTrap™ protein G HP
Supplier: Cytiva
HiTrap™ Protein G HP affinity columns are pre-packed with 1 or 5 ml of Protein G Sepharose™ high performance for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.
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Affinity chromatography columns, StrepTrap™ XT
Supplier: Cytiva
A chromatography resin for the purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®, which provides higher affinity, resulting in higher yields and lower loss of valuable target protein.
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Western blotting detection reagent, Amersham™ ECL Select™
Supplier: Cytiva
Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.
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Amersham™ Protran® Supported Nitrocellulose Western Blotting Membranes, Cytiva
Supplier: Cytiva
Amersham™ Protran® Supported is a robust protein blotting membrane, with 0,2 µm pore size, made of reinforced nitrocellulose for multiple reprobings.
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