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Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva

Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Calmodulin Sepharose™ 4B is calmodulin immobilised by the CNBr method to Sepharose™ 4B. Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells. This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD⁺/NADP⁺.

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Cation exchange chromatography column, Mono S HR

Supplier: Cytiva

Mono S HR is a strong cation exchanger prepacked with MonoBeads™. The column is an excellent choice for the polishing step in purification of proteins, peptides, and other biomolecules when high purity is required.

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Anion exchange chromatography column, Mono Q HR

Supplier: Cytiva

Anion exchange chromatography column, Mono Q HR

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Anion exchange chromatography columns, HiTrap™ DEAE FF

Anion exchange chromatography columns, HiTrap™ DEAE FF

Supplier: Cytiva

HiTrap DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

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Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Supplier: Cytiva

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

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Accessories for HiTrap™ columns

Accessories for HiTrap™ columns

Supplier: Cytiva

Union M6 female, 1/16" male, PEEK

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Combs for vertical electrophoresis systems, SE 600 and SE 400

Combs for vertical electrophoresis systems, SE 600 and SE 400

Supplier: Cytiva

Note that the comb in the picture has the same number of wells and well width (8.3 mm), but differ regarding the t-value.

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Affinity coupling media, EAH Sepharose™ 4B

Affinity coupling media, EAH Sepharose™ 4B

Supplier: Cytiva

EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.

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Blotting membranes, Amersham™ Hybond™ ECL™

Blotting membranes, Amersham™ Hybond™ ECL™

Supplier: Cytiva

Hybond™ ECL™ gives excellent sensitivity, resolution, and low background for all labelling and detection systems (radioactive, non radioactive, and chromogenic), especially when used with ECL™, ECL™ Prime, and ECL™ Plex Western Blotting Detection kits and reagents. Special packaging features include interleaf on both sides of the membrane and air-tight resealable aluminium bag with label space for marking the 'opened on' date.

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High purity dNTPs

High purity dNTPs

Supplier: Cytiva

dNTPs designed for applications requiring high purity such as amplification, dideoxy sequencing, labeling, mutagenesis, cDNA synthesis, and expression profiling.

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Autoradiography cassettes, Amersham Hypercassette™

Autoradiography cassettes, Amersham Hypercassette™

Supplier: Cytiva

Robust autoradiography cassettes for light-tight exposures at temperatures down to –70 °C.

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Sodium dodecyl sulphate (SDS), PlusOne™

Sodium dodecyl sulphate (SDS), PlusOne™

Supplier: Cytiva

SDS is an ionic detergent used to solubilise proteins. The PlusOne™ SDS reagent is carefully controlled for carbon chain length and overall quality.

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Sepharose™ 6B Gel Filtration Base Matrix

Sepharose™ 6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 6B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Blue Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva

Blue Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

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Precast gels, CleanGel IEF

Supplier: Cytiva

CleanGel IEF is a dried polyacrylamide gel optimised for analytical isoelectric focusing (IEF). The gel is excellent for separating enzymes with preserved activity.

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Anti-IgG Sheep Antibody (HRP (Horseradish Peroxidase))

Anti-IgG Sheep Antibody (HRP (Horseradish Peroxidase))

Supplier: Cytiva

For use in ELISA systems
For ELISA, it is recommended that the conjugate should be diluted in the range 1:4000 to 1:8000
Amdex conjugates are already supplied as components of the Biotrak high-sensitivity cytokine ELISAs, complete with fully-optimized protocols
Easily soluble in cold and hot water

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MicroSpin™ DNA Clean-up, Columns

MicroSpin™ DNA Clean-up, Columns

Supplier: Cytiva

MicroSpin™ columns are designed for the rapid purification of DNA in conjunction with Sephadex™ G-25 DNA grade for use in a wide range of applications, including desalting, buffer exchange and removal of unincorporated nucleotides from end-labelled oligonucleotides.

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Deaza dGTP

Deaza dGTP

Supplier: Cytiva

Band compressions are ambiguous band patterns on sequencing gels caused by stable intra-strand secondary structures that are not fully denatured during electrophoresis. Their occurrence can be reduced by substituting nucleotide analogues in the sequencing mixes. 7-Deaza dGTP (c⁷dGTP) can be used to resolve G-band compressions.

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Chart recorder pens

Supplier: Cytiva

Pens for use with paper chart recorders.

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PhastTransfer™ Kit

Supplier: Cytiva

PhastTransfer™ Kit

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Ion exchange chromatography media, SOURCE™ 30S

Ion exchange chromatography media, SOURCE™ 30S

Supplier: Cytiva

SOURCE 30S is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix It is modified with sulphonate (S) strong cation exchange groups.

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DALT Buffer kit for precast gels

Supplier: Cytiva

DALT Buffer kit is used together with DALT Gel 12,5, which comprises concentrated buffers for running the gel, gel buffer for inserting the gel into DALT precast gel cassette and sealing solution for application of the IPG strip. The buffer in the gel is part of a buffer system that gives longer shelf-life.

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Training Booklet and Reagent Kit for Biacore T200

Supplier: Cytiva

Hands-on system operation and basic methodology for new users.

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HiTrap™ Fibro™ PrismA Affinity Chromatography Columns

Supplier: Cytiva

PrismA are protein A fibre chromatography units for rapid cycling chromatography purification of mAbs for research and process development applications.

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Filter plates, 96-well, PD MultiTrap™ G-25

Filter plates, 96-well, PD MultiTrap™ G-25

Supplier: Cytiva

PD MultiTrap G-25 is designed for high-throughput desalting and buffer exchange of multiple samples.

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Mercury lamps

Supplier: Cytiva

Mercury lamp and housing, UPC-900

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Tricorn Empty Chromatography Columns

Tricorn Empty Chromatography Columns

Supplier: Cytiva

Tricorn empty columns are for high resolution small-scale chromatography at pressures up to 50 bar (Tricorn 10) or 100 (Tricorn 5).

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Ion exchange chromatography media, Capto™ Q ImpRes

Ion exchange chromatography media, Capto™ Q ImpRes

Supplier: Cytiva

Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

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Spin Adapters for Gravity Columns

Spin Adapters for Gravity Columns

Supplier: Cytiva

Convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel, High desalting capacity, The use of centrifugation protocol with all gravity columns.

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Affinity chromatography columns, HiTrap™ Protein A HP

Affinity chromatography columns, HiTrap™ Protein A HP

Supplier: Cytiva

HiTrap™ Protein A HP is a pre-packed, ready to use Protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum and cell culture supernatants.

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