HiLoad Superdex columns, 75 PG
Supplier: Cytiva
Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component
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Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media
Supplier: Cytiva
Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.
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illustra™ TempliPhi™100/500 Amplification Kits
Supplier: Cytiva
TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.
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Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow
Supplier: Cytiva
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
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Affinity coupling media, CNBr-Activated Sepharose™ 4B
Supplier: Cytiva
CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
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Affinity chromatography columns, HisTrap™ HP
Supplier: Cytiva
HisTrap HP prepacked columns provide a fast, convenient and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.
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Anion exchange chromatography columns, Mono Q™
Supplier: Cytiva
Mono Q™ columns are strong anion exchange chromatography columns for protein analysis or small scale, high resolution polishing of proteins.
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Sample concentrators, Vivaspin®
Supplier: Cytiva
Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.
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Anti-IgG Sheep Polyclonal Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
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Columns, PD MidiTrap™ G-25
Supplier: Cytiva
PD MidiTrap G-25 is convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel, The use of centrifugation protocol with all gravity columns.
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Affinity chromatography columns, His GraviTrap™
Supplier: Cytiva
His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.
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Accessories for empty columns XK series
Supplier: Cytiva
Ferrules for XK 16 and XK 26
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Gel filtration media, Sephadex™ G-50
Supplier: Cytiva
Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.
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Amersham™ Protran® Supported Nitrocellulose Western Blotting Membranes, Cytiva
Supplier: Cytiva
Amersham™ Protran® Supported is a robust protein blotting membrane, with 0,2 µm pore size, made of reinforced nitrocellulose for multiple reprobings.
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Desalting columns with Sephadex G-25 resin, HiTrap™
Supplier: Cytiva
HiTrap Desalting are ready-to-use 5 ml columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange.
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Desalting columns, HiPrep™
Supplier: Cytiva
HiPrep™ 26/10 desalting column is a convenient, easy to use column pre-packed with Sephadex™ G-25 Fine for desalting and buffer exchange with sample sizes of up to 15 ml per column.
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Empty disposable columns, PD-10
Supplier: Cytiva
PD-10 Columns are economical, plastic columns for gravity flow purification.
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Affinity chromatography columns, HiTrap™ protein G HP
Supplier: Cytiva
HiTrap™ Protein G HP affinity columns are pre-packed with 1 or 5 ml of Protein G Sepharose™ high performance for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.
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Protease affinity chromatography media, PreScission™, Cytiva
Supplier: Cytiva
Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.
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Cation exchange chromatography columns, HiTrap™ SP FF
Supplier: Cytiva
HiTrap™ SP FF is pre-packed with SP Sepharose™ Fast Flow, and is a strong cation exchanger for small scale protein purifications as well as screening of binding and elutions conditions.
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Precast ExcelGel for SDS-PAGE
Supplier: Cytiva
ExcelGel precast gels for SDS-PAGE are available as homogeneous or gradient gels. The homogeneous gels have 25 preformed sample wells for sample volumes from 5 to 17 µl. The gradient gels use sample application strips (26 samples up to 40 µl, 52 samples up to 20 µl), paper pieces or sample droplets. All ExcelGel SDS homogeneous and 8 - 18 gradient gels have a 33 mm stacking zone and a 77 mm separating zone.
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DNA clean up, Sephadex® G-50 DNA Grade F
Supplier: Cytiva
Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.
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Western blotting detection reagent, Amersham™ ECL Select™
Supplier: Cytiva
Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.
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Blotting membranes, Amersham™ Hybond™ 0,45 PVDF
Supplier: Cytiva
Amersham™ Hybond™ P 0,45 PVDF is a 0,45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. For use in chemiluminescent and fluorescent detection methods for proteins of >Mr 20000.
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illustra™ ExoProStar™ 1-STEP kits
Supplier: Cytiva
illustra™ ExoProStar™ 1-STEP Kit uses illustra™ Exonuclease I and Alkaline Phosphatase for optimal removal of unincorporated primers and nucleotides. illustra™ ExoProStar™ improves digestion efficiency with no degradation of the target PCR product. The sample remains completely intact and ready for immediate use in manual or automated processes.
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Affinity chromatography columns, HisTrap™ FF
Supplier: Cytiva
HisTrap™ FF columns are designed for simple, one-step purification and scaling up purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose™ 6 Fast Flow.
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Affinity chromatography media, Ni Sepharose™ 6 Fast Flow
Supplier: Cytiva
Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.
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Size exclusion chromatography columns, illustra™ NAP™
Supplier: Cytiva
NAP™ columns are disposable columns prepacked with Sephadex™ G-25 DNA grade and require only gravity to run.
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Anti-IgG Sheep Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
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Affinity chromatography columns, HiTrap™ NHS-Activated HP
Supplier: Cytiva
HiTrap NHS-activated HP prepacked chromatography columns allow you to design an affinity column suitable for your purification needs.