Fraction collector, Frac30
Supplier: Cytiva
Frac30 is a round fraction collector designed for use with the ÄKTA start purification system. The Frac30 is controlled via the ÄKTA start touch screen instrument display or using UNICORN start software on a computer. The Frac30 is attached to ÄKTA start for power and communication. It is not suited for standalone operation.
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Western blotting detection reagent, Amersham™ ECL™ Prime
Supplier: Cytiva
Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.
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Accessories for empty columns HiScale™
Supplier: Cytiva
Union Valco F/F, PEEK, for HiScale™ 16 and 26
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Gel filtration media, Sephadex™ LH-20
Supplier: Cytiva
Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.
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illustra™ MicroSpin™ S-300 HR columns
Supplier: Cytiva
illustra™ MicroSpin™ S-300 HR columns designed for rapid purification of PCR products (>100 bp) from unincorporated primers (<20-mers) and nucleotides using spin-column chromatography.
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Accessories for empty high performance columns Tricorn™
Supplier: Cytiva
Fingertight connector 1/16" male
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Percoll™
Supplier: Cytiva
Percoll® is commonly used for separating cells, organelles, viruses, and other subcellular particles.
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Blotting membranes, Amersham™ Hybond™ 0.2 PVDF
Supplier: Cytiva
Amersham™ Hybond™ 0.2 PVDF is a 0,2 μm pore size hydrophobic membrane with high physical strength. This results in significant handling advantages over unsupported nitrocellulose and makes the membrane highly suitable for stripping and reprobing. The small pore size of the membrane minimises 'blow-through' and increases protein binding over a wide range of molecular weights. Ideal for use with standard colorimetric and chemiluminescent detection methods for proteins of
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Pharmalytes® IEF carrier ampholytes
Supplier: Cytiva
Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
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Agarose IEF
Supplier: Cytiva
Agarose with low electroendosmosis (EEO) is recommended for most DNA/RNA applications.
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pI markers
Supplier: Cytiva
pI markers are lyophilized mixtures of stable, salt-free, highly purified proteins.
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Affinity chromatography media, Streptavidin Sepharose™ High Performance
Supplier: Cytiva
Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.
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Anion exchange chromatography columns, HiTrap Q HP
Supplier: Cytiva
HiTrap® Q HP are prepacked, ready-to-use High Performance strong anion exchange columns for high-resolution, small-scale protein purification.
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dNTP sets, illustra™
Supplier: Cytiva
High purity dNTPs for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.
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Spin columns, Protein A HP SpinTrap™
Supplier: Cytiva
Protein A HP SpinTrap™ columns are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids.
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Protein and peptide molecular weight markers
Supplier: Cytiva
For determination of molecular weight of uncharacterised protein samples with precision by comparison with carefully selected standards.
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Anti-IgG Sheep Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
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Sera-Mag carboxylate-modified magnetic beads and SpeedBeads
Supplier: Cytiva
Sera-Mag Carboxylate-Modified Magnetic Beads & SpeedBeads have free carboxyl groups on the surface for convenient covalent coupling of target molecules (proteins, peptides, and amine-modified ligands) via primary amines (NH2).
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Ficoll-Paque® PLUS and Ficoll-Paque® PREMIUM Centrifugation Media, Cytiva
Supplier: Cytiva
Ficoll-Paque™ PREMIUM products are a range of sterile, ready to use density gradient media for the preparation of mononuclear cells.
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illustra™ ExoProStar™ 1-STEP kits
Supplier: Cytiva
illustra™ ExoProStar™ 1-STEP Kit uses illustra™ Exonuclease I and Alkaline Phosphatase for optimal removal of unincorporated primers and nucleotides. illustra™ ExoProStar™ improves digestion efficiency with no degradation of the target PCR product. The sample remains completely intact and ready for immediate use in manual or automated processes.
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Affinity chromatography columns, HiTrap™ NHS-Activated HP
Supplier: Cytiva
HiTrap NHS-activated HP prepacked chromatography columns allow you to design an affinity column suitable for your purification needs.
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Size exclusion chromatography columns, illustra™ NAP™
Supplier: Cytiva
NAP™ columns are disposable columns prepacked with Sephadex™ G-25 DNA grade and require only gravity to run.
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Blotting membranes, Amersham™ Hybond™ 0,45 PVDF
Supplier: Cytiva
Amersham™ Hybond™ P 0,45 PVDF is a 0,45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. For use in chemiluminescent and fluorescent detection methods for proteins of >Mr 20000.
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illustra™ ProbeQuant™ G-50 micro columns
Supplier: Cytiva
ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.
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Affinity chromatography columns, HisTrap™ FF
Supplier: Cytiva
HisTrap™ FF columns are designed for simple, one-step purification and scaling up purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose™ 6 Fast Flow.
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Affinity chromatography media, Ni Sepharose™ 6 Fast Flow
Supplier: Cytiva
Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.
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Columns, PD MiniTrap™ G-25
Supplier: Cytiva
PD MiniTrap G-25 columns are prepacked, single-use gravity columns for desalting, buffer exchange, and sample cleanup.
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Columns, PD SpinTrap™ G-25
Supplier: Cytiva
PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules.
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Amersham™ Protran® Western Blotting Membranes, Nitrocellulose
Supplier: Cytiva
Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.
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HiLoad Superdex columns, 75 PG
Supplier: Cytiva
Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component