"Biowest"

Supplier: Biowest

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

Supplier: Biowest

Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

Supplier: Biowest

Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

Supplier: Biowest

Glutamine penicillin streptamycin acts against gram-negative and gram-positive bacteria.

Supplier: Biowest

Dulbecco's Modified Eagle Medium (DMEM) is a modification of Basal Medium Eagle (BME) which contains approximately four times as much of the vitamins and amino acids, and two to four times as much glucose (depending on the version) as the original formulation.

Supplier: Biowest

Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

Supplier: Biowest

Lymphosep is a standardised, high quality medium designed for simple and rapid isolation of PBMCs from treated whole blood. Lymphosep, like Ficoll™, has the optimised density 1,077 g/ml for lymphocyte separation to guarantee best and reliable results for your preparations.

Supplier: Biowest

Penicillin-Streptomycin acts against Gram-negative and Gram-positive bacteria.

Supplier: Biowest

L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

Supplier: Biowest

The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Supplier: Biowest

Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, Drosophila melanogaster. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

Supplier: Biowest

Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.

Supplier: Biowest

L(+)-Glutamine 0.2 mol/l in aqueous solution

Supplier: Biowest

Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

Supplier: Biowest

Amphotericin B used in tissue culture to prevent growth of yeasts and fungi. It changes the function and integrity of eucaryotic cell membranes by forming complexes with sterols (cholesterol) hence having no effect on bacteria. Cause leakage of glucose.

Supplier: Biowest

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.